Nitric oxide synthase generates nitric oxide locally to regulate compartmentalized protein S-nitrosylation and protein trafficking.

Nitric oxide synthase generates nitric oxide locally to regulate compartmentalized protein S-nitrosylation and protein trafficking. Research Abstract Details 

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Nitric oxide synthase generates nitric oxide locally to regulate compartmentalized protein S-nitrosylation and protein trafficking. Abstract Text:

Yasuko Iwakiri,Ayano Satoh,Suvro Chatterjee,Derek K Toomre,Cecile M Chalouni,David Fulton,Roberto J Groszmann,Vijay H Shah,William C Sessa,

Nitric oxide (NO) is a highly diffusible and short-lived physiological messenger. Despite its diffusible nature, NO modifies thiol groups of specific cysteine residues in target proteins and alters protein function via S-nitrosylation. Although intracellular S-nitrosylation is a specific posttranslational modification, the defined localization of an NO source (nitric oxide synthase, NOS) with protein S-nitrosylation has never been directly demonstrated. Endothelial NOS (eNOS) is localized mainly on the Golgi apparatus and in plasma membrane caveolae. Here, we show by using eNOS targeted to either the Golgi or the nucleus that S-nitrosylation is concentrated at the primary site of eNOS localization. Furthermore, localization of eNOS on the Golgi enhances overall Golgi protein S-nitrosylation, the specific S-nitrosylation of N-ethylmaleimide-sensitive factor and reduces the speed of protein transport from the endoplasmic reticulum to the plasma membrane in a reversible manner. These data indicate that local NOS action generates organelle-specific protein S-nitrosylation reactions that can regulate intracellular transport processes.

Nitric oxide synthase generates nitric oxide locally to regulate compartmentalized protein S-nitrosylation and protein trafficking. Publishing Authors By Initials

Y Iwakiri,A Satoh,S Chatterjee,DK Toomre,CM Chalouni,D Fulton,RJ Groszmann,VH Shah,WC Sessa,

For similar s-nitrosothiols research abstracts see: s-nitrosothiols research

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Nitric oxide synthase generates nitric oxide locally to regulate compartmentalized protein S-nitrosylation and protein trafficking. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 103

Page Numbers: 19777-82

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 14

MONTH: 12

YEAR: 2006

Nitric oxide synthase generates nitric oxide locally to regulate compartmentalized protein S-nitrosylation and protein trafficking. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Nitric oxide synthase generates nitric oxide locally to regulate compartmentalized protein S-nitrosylation and protein trafficking. Keywords Mesh Terms:

KEYWORDS: S-Nitrosothiols

MESH TERMS: metabolism

Chemical & Substance for Abstract: Nitric oxide synthase generates nitric oxide locally to regulate compartmentalized protein S-nitrosylation and protein trafficking. Information

Substance Name: Nitric Oxide Synthase Type III

Registry Number: EC 1.14.13.39

Grant and Affiliation Information for Nitric oxide synthase generates nitric oxide locally to regulate compartmentalized protein S-nitrosylation and protein trafficking.

AFFILIATION: Section of Digestive Diseases, Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06510, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: R01 HL64793

ACRONYM: HL

MEDLINETA: Proc Natl Acad Sci U S A

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