Nicotinamidase activity is important for germination.

Nicotinamidase activity is important for germination. Research Abstract Details 

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Nicotinamidase activity is important for germination. Abstract Text:

Lee Hunt,Michael J Holdsworth,Julie E Gray,

It has been suggested that nicotinamide must be degraded during germination; however, the enzyme responsible and its physiological role have not been previously studied. We have identified an Arabidopsis gene, NIC2, that is expressed at relatively high levels in mature seed, and encodes a nicotinamidase enzyme with homology to yeast and bacterial nicotinamidases. Seed of a knockout mutant, nic2-1, had reduced nicotinamidase activity, retarded germination and impaired germination potential. nic2-1 germination was restored by after-ripening or moist chilling, but remained hypersensitive to application of nicotinamide or ABA. Nicotinamide is a known inhibitor of NAD-degrading poly(ADP-ribose) polymerases (PARP enzymes) that are implicated in DNA repair. We found reduced poly(ADP)ribosylation levels in nic2-1 seed, which were restored by moist chilling. Furthermore, nic2-1 seed had elevated levels of NAD, and germination was hypersensitive to methyl methanesulphonate (MMS), suggesting that PARP activity and DNA repair responses were impaired. We suggest that nicotinamide is normally metabolized by NIC2 during moist chilling or after-ripening, which relieves inhibition of PARP activity and allows DNA repair to occur prior to germination.

Nicotinamidase activity is important for germination. Publishing Authors By Initials

L Hunt,MJ Holdsworth,JE Gray,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, amino acid research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, amino acid research

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Nicotinamidase activity is important for germination. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Plant journal : for cell and molecular biology

VOLUME: 51

Page Numbers: 341-51

Journal Abbreviation: Plant J.

ISSN: 0960-7412

DAY: 22

MONTH: 06

YEAR: 2007

Nicotinamidase activity is important for germination. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9207397

Nicotinamidase activity is important for germination. Keywords Mesh Terms:

KEYWORDS: Sequence Homology, Amino Acid

MESH TERMS: metabolism

Chemical & Substance for Abstract: Nicotinamidase activity is important for germination. Information

Substance Name: Nicotinamidase

Registry Number: EC 3.5.1.19

Grant and Affiliation Information for Nicotinamidase activity is important for germination.

AFFILIATION: Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK.

Country: England

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MEDLINETA: Plant J

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