New radioisotopic assays of argininosuccinate synthetase and argininosuccinase.

New radioisotopic assays of argininosuccinate synthetase and argininosuccinase. Research Abstract Details 

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New radioisotopic assays of argininosuccinate synthetase and argininosuccinase. Abstract Text:

H Kato,I Oyamada,M Mizutani-Funahashi,H Nakagawa,

Methods were developed for the radioisotopic assay of argininosuccinate synthetase [L-citrulline: L-aspartate ligase (AMP-forming), EC 6.3.4.5] and argininosuccinase [L-argininosuccinate arginine-lyase, EC 4.3.2.1]. The assay of argininosuccinate synthetase was based on the separation of [14C]argininosuccinate formed from aspartate and [carbamoyl-14C]citrulline in the presence of ATP from the substrate citrulline. For this, the product was converted to its anhydride form by boiling for 30 min at pH 2.0 followed by application on a column of Dowex 50W (pyridine form). Argininosuccinic anhydride was eluted with 0.3 M pyridine acetate buffer, pH 4.25, while citrulline was eluted with 0.1 M pyridine acetate buffer, pH 3.80. The assay of argininosuccinase was based on the separation of [14C]argininosuccinic acid formed from arginine and [U-14C]fumaric acid from the substrate fumarate on a column of Dowex 50W(H+ form). The argininosuccinic acid was adsorbed on the column and eluted with 1 M pyridine solution, while fumarate was not adsorbed. The distributions of these two enzymes in various organs and cell fractions were reinvestigated using these methods.

New radioisotopic assays of argininosuccinate synthetase and argininosuccinase. Publishing Authors By Initials

H Kato,I Oyamada,M Mizutani-Funahashi,H Nakagawa,

For similar cells: cellular structures: subcellular fractions research abstracts see: cells: cellular structures: subcellular fractions research

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New radioisotopic assays of argininosuccinate synthetase and argininosuccinase. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 79

Page Numbers: 945-53

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1976

New radioisotopic assays of argininosuccinate synthetase and argininosuccinase. Information

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LANGUAGE: eng

NlmUniqueID: 376600

New radioisotopic assays of argininosuccinate synthetase and argininosuccinase. Keywords Mesh Terms:

KEYWORDS: Subcellular Fractions

MESH TERMS: enzymology

Chemical & Substance for Abstract: New radioisotopic assays of argininosuccinate synthetase and argininosuccinase. Information

Substance Name: Argininosuccinate Synthase

Registry Number: EC 6.3.4.5

Grant and Affiliation Information for New radioisotopic assays of argininosuccinate synthetase and argininosuccinase.

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Country: JAPAN

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MEDLINETA: J Biochem

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