New method to prepare single-headed heavy meromyosin with high purity and a high yield.

New method to prepare single-headed heavy meromyosin with high purity and a high yield. Research Abstract Details 

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New method to prepare single-headed heavy meromyosin with high purity and a high yield. Abstract Text:

K Saeki,M Tokunaga,H A Ting,T Wakabayashi,

We have developed a new method to prepare single-headed heavy meromyosin with high purity and a high yield. To examine whether the two heads on the same myosin molecule work cooperatively or not, it is important to prepare pure single-headed heavy meromyosin. Myosin was extracted from myofibrils treated with a solution containing CyDTA, a strong divalent cation chelator. CyDTA treatment was essential to the production of sHMM. Then such myosin was digested with chymotrypsin in the presence of divalent cations at high ionic strength. Crude sHMM was separated from double-headed HMM by affinity chromatography using an ADP-column. Contaminating S1 was removed by gel filtration. Heavy chain of sHMM obtained by the present method had no nick. Purified sHMM showed normal EDTA-ATPase and Ca-ATPase. It interacted with thin filament and its ATPase was activated by actin normally.

New method to prepare single-headed heavy meromyosin with high purity and a high yield. Publishing Authors By Initials

K Saeki,M Tokunaga,HA Ting,T Wakabayashi,

For similar animals: chordata: vertebrates: mammals: lagomorpha: rabbits research abstracts see: animals: chordata: vertebrates: mammals: lagomorpha: rabbits research

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New method to prepare single-headed heavy meromyosin with high purity and a high yield. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 106

Page Numbers: 606-11

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1989

New method to prepare single-headed heavy meromyosin with high purity and a high yield. Information

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LANGUAGE: eng

NlmUniqueID: 376600

New method to prepare single-headed heavy meromyosin with high purity and a high yield. Keywords Mesh Terms:

KEYWORDS: Rabbits

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: New method to prepare single-headed heavy meromyosin with high purity and a high yield. Information

Substance Name: Adenosine Triphosphatases

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for New method to prepare single-headed heavy meromyosin with high purity and a high yield.

AFFILIATION: Department of Physics, Faculty of Science, University of Tokyo.

Country: JAPAN

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MEDLINETA: J Biochem

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