New insights into the biosynthesis of mycobacterial lipomannan arising from deletion of a conserved gene.

New insights into the biosynthesis of mycobacterial lipomannan arising from deletion of a conserved gene. Research Abstract Details 

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New insights into the biosynthesis of mycobacterial lipomannan arising from deletion of a conserved gene. Abstract Text:

Devinder Kaur,Michael R McNeil,Kay-Hooi Khoo,Delphi Chatterjee,Dean C Crick,Mary Jackson,Patrick J Brennan,

Genetic construction of a mutant strain (designated MSMEG4245) of Mycobacterium smegmatis, defective in a broadly conserved gene for a putative glycosyltransferase of the glycosyltransferase-C superfamily, results in a phenotype marked by the virtual absence of the phosphatidylinositol-containing lipomannan and lipoarabinomannan, replaced instead by a novel truncated form of lipomannan. The normal spectrum of phosphatidylinositol mannosides, long presumed precursors of these lipoglycans, was retained. Matrix-assisted laser desorption/ionization-time of flight/mass spectrometry of the mutated form of lipomannan shows a family of phosphatidylinositol-anchored lipomannans with from only 5 to 20 Manp residues as compared with lipomannan from the wild type strain consisting of 21-34 Manp residues but with few changes in the branching pattern. Thus, MSMEG4245 is apparently a key mannosyltransferase, required for the proper elongation of lipomannan to its normal state and subsequent synthesis of lipoarabinomannan. The corresponding ortholog in Mycobacterium tuberculosis H37Rv has been identified as Rv2174. This previously unrecognized feature of the biosynthesis of lipomannan/lipoarabinomannan allows a significant revision of structural and biosynthetic schemata and provides a molecular basis of selectivity in biosynthesis, as conferred by the MSMEG4245 gene.

New insights into the biosynthesis of mycobacterial lipomannan arising from deletion of a conserved gene. Publishing Authors By Initials

D Kaur,MR McNeil,KH Khoo,D Chatterjee,DC Crick,M Jackson,PJ Brennan,

For similar bacteria: gram-positive bacteria: actinobacteria: actinomycetales: mycobacteriaceae: mycobacterium: mycobacteria, atypical: mycobacterium smegmatis research abstracts see: bacteria: gram-positive bacteria: actinobacteria: actinomycetales: mycobacteriaceae: mycobacterium: mycobacteria, atypical: mycobacterium smegmatis research

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New insights into the biosynthesis of mycobacterial lipomannan arising from deletion of a conserved gene. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 27133-40

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 2

MONTH: 07

YEAR: 2007

New insights into the biosynthesis of mycobacterial lipomannan arising from deletion of a conserved gene. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

New insights into the biosynthesis of mycobacterial lipomannan arising from deletion of a conserved gene. Keywords Mesh Terms:

KEYWORDS: Mycobacterium smegmatis

MESH TERMS: metabolism

Chemical & Substance for Abstract: New insights into the biosynthesis of mycobacterial lipomannan arising from deletion of a conserved gene. Information

Substance Name: Glycosyltransferases

Registry Number: EC 2.4.-

Grant and Affiliation Information for New insights into the biosynthesis of mycobacterial lipomannan arising from deletion of a conserved gene.

AFFILIATION: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI 18357

ACRONYM: AI

MEDLINETA: J Biol Chem

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