New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase.

New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase. Research Abstract Details 

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New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase. Abstract Text:

T Morita,H Kato,S Iwanaga,K Takada,T Kimura,

Twenty peptide-4-methylcoumarin amides (MCA) were newly synthesized and tested as possible substrates for alpha-thrombin, factor Xa, kallikreins, urokinase, and plasmin. These fluorogenic peptides contained arginine-MCA as the carboxyl-terminus. Release of 7-amino-4-methylcoumarin was determined fluorometrically. Of these peptides, the following were found to be specific substrates for individual enzymes: Boc-Val-Pro-Arg-MCA for alpha-thrombin, Boc-Ile-Glu-Gly-Arg-MCA, and Boc-Ser-Gly-Arg-MCA for factor Xa, Z-Phe-Arg-MCA for plasma kallikrein, Pro-Phe-Arg-MCA for pancreatic and urinary kallikreins, and glutaryl-Gly-Arg-MCA for urokinase. Moreover, these peptide-MCA substrates were resistant to plasmin.

New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase. Publishing Authors By Initials

T Morita,H Kato,S Iwanaga,K Takada,T Kimura,

For similar enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: urinary plasminogen activator research abstracts see: enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: urinary plasminogen activator research

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New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 82

Page Numbers: 1495-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1977

New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase. Keywords Mesh Terms:

KEYWORDS: Urinary Plasminogen Activator

MESH TERMS: metabolism

Chemical & Substance for Abstract: New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase. Information

Substance Name: Urinary Plasminogen Activator

Registry Number: EC 3.4.21.73

Grant and Affiliation Information for New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase.

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Country: JAPAN

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MEDLINETA: J Biochem

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