Neurotrophic and growth factor gene expression profiling of mouse bone marrow stromal cells induced by ischemic brain extracts.

Neurotrophic and growth factor gene expression profiling of mouse bone marrow stromal cells induced by ischemic brain extracts. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Neurotrophic and growth factor gene expression profiling of mouse bone marrow stromal cells induced by ischemic brain extracts. Abstract Text:

Runjiang Qu,Yi Li,Qi Gao,Lihong Shen,Jing Zhang,Zhongwu Liu,Xiaoguang Chen,Michael Chopp,

Treatment of rodents after stroke with bone marrow stromal cells (BMSCs) improves functional outcome. However, the mechanisms underlying this benefit have not been ascertained. This study focused on the contribution of neurotrophic and growth factors produced by BMSCs to therapeutic benefit. Rats were subjected to middle cerebral artery occlusion and the ischemic brain extract supernatant was collected to prepare the conditioned medium. The counterpart normal brain extract from non-ischemic rats was employed as the experimental control. Using microarray assay, we measured the changes of the neurotrophin associated gene expression profile in BMSCs cultured in different media. Furthermore, real-time RT-PCR and fluorescent immunocytochemistry were utilized to validate the gene changes. The morphology of BMSCs, cultured in the ischemic brain-conditioned medium for 12 h, was dramatically altered from a polygonal and flat appearance to a fibroblast-like long and thin cell appearance, compared to those in the normal brain-conditioned medium and the serum replacement medium. Forty-four neurotrophin-associated genes in BMSCs were identified by microarray assay under all three culture media. Twelve out of the 44 genes (7 neurotrophic and growth factor genes, 5 receptor genes) increased in BMSCs cultured in the ischemic brain-conditioned medium compared to the normal brain-conditioned medium. Real time RT-PCR and immunocytochemistry validated that the ischemic brain-conditioned medium significantly increased 6/7 neurotrophic and growth factor genes, compared with the normal brain-conditioned medium. These six genes consisted of fibroblast growth factor 2, insulin-like growth factor 1, vascular endothelial growth factor A, nerve growth factor beta, brain-derived neurotrophic factor and epidermal growth factor. Our results indicate that transplanted BMSCs may work as 'small molecular factories' by secreting neurotrophins, growth factors and other supportive substances after stroke, which may produce therapeutic benefits in the ischemic brain.

Neurotrophic and growth factor gene expression profiling of mouse bone marrow stromal cells induced by ischemic brain extracts. Publishing Authors By Initials

R Qu,Y Li,Q Gao,L Shen,J Zhang,Z Liu,X Chen,M Chopp,

For similar complex mixtures: tissue extracts research abstracts see: complex mixtures: tissue extracts research

PUBMED ID PMID:

MEDLINE DATE:

Neurotrophic and growth factor gene expression profiling of mouse bone marrow stromal cells induced by ischemic brain extracts. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Neuropathology : official journal of the Japanese

VOLUME: 27

Page Numbers: 355-63

Journal Abbreviation:

ISSN: 0919-6544

DAY: 3

MONTH: Aug

YEAR: 2007

Neurotrophic and growth factor gene expression profiling of mouse bone marrow stromal cells induced by ischemic brain extracts. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9606526

Neurotrophic and growth factor gene expression profiling of mouse bone marrow stromal cells induced by ischemic brain extracts. Keywords Mesh Terms:

KEYWORDS: Tissue Extracts

MESH TERMS: metabolism

Chemical & Substance for Abstract: Neurotrophic and growth factor gene expression profiling of mouse bone marrow stromal cells induced by ischemic brain extracts. Information

Substance Name: Tissue Extracts

Registry Number: 0

Grant and Affiliation Information for Neurotrophic and growth factor gene expression profiling of mouse bone marrow stromal cells induced by ischemic brain extracts.

AFFILIATION: Department of Neurology, Henry Ford Health System, Detroit, Michigan, USA.

Country: Australia

AGENCY: United States NINDS

GRANT: P01 NS23393

ACRONYM: NS

MEDLINETA: Neuropathology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Neurotrophic and growth factor gene expression profiling of mouse bone marrow stromal cells induced by ischemic brain extracts Related Publications

• Atorvastatin promotes presenilin-1 expression and notch1 activity and increases neural progenitor cell proliferation after stroke.
• Bone marrow stromal cells induce BMP2/4 production in oxygen-glucose-deprived astrocytes, which promotes an astrocytic phenotype in adult subventricular progenitor cells.
• Collagen scaffolds populated with human marrow stromal cells reduce lesion volume and improve functional outcome after traumatic brain injury.
• Combination of surgical resection and photodynamic therapy of 9L gliosarcoma in the nude rat.
• Combination therapy with antiangiogenic treatment and photodynamic therapy for the nude mouse bearing u87 glioblastoma.
• Comparison of in vivo and in vitro gene expression profiles in subventricular zone neural progenitor cells from the adult mouse after middle cerebral artery occlusion.
• Coupling of angiogenesis and neurogenesis in cultured endothelial cells and neural progenitor cells after stroke.
• Decrease of endogenous vascular endothelial growth factor may not affect glioma cell proliferation and invasion.
• Ectopic doublecortin gene expression suppresses the malignant phenotype in glioblastoma cells.
• Ectopic expression of doublecortin protects adult rat progenitor cells and human glioma cells from severe oxygen and glucose deprivation.
• Evaluation of corticospinal axon loss by fluorescent dye tracing in mice with experimental autoimmune encephalomyelitis.
• Gene profiles within the adult subventricular zone niche: proliferation, differentiation and migration of neural progenitor cells in the ischemic brain.
• Inhibition of ADAM17 reduces hypoxia-induced brain tumor cell invasiveness.
• Ischemic rat brain extracts induce human marrow stromal cell growth factor production.
• Lengthening the G(1) phase of neural progenitor cells is concurrent with an increase of symmetric neuron generating division after stroke.
• Measurement of neuronal activity of individual neurons after stroke in the rat using a microwire electrode array.
• Multiparametric iterative self-organizing MR imaging data analysis technique for assessment of tissue viability in acute cerebral ischemia.
• Neuroblast division during migration toward the ischemic striatum: a study of dynamic migratory and proliferative characteristics of neuroblasts from the subventricular zone.
• Neurotrophic and growth factor gene expression profiling of mouse bone marrow stromal cells induced by ischemic brain extracts.
• Nitric oxide donor upregulation of stromal cell-derived factor-1/chemokine (CXC motif) receptor 4 enhances bone marrow stromal cell migration into ischemic brain after stroke.
• Post-acute response of 9L gliosarcoma to Photofrin-mediated PDT in athymic nude mice.
• Role of gender in outcome after traumatic brain injury and therapeutic effect of erythropoietin in mice.
• Simvastatin-Mediated Upregulation of VEGF and BDNF, Activation of the PI3K/Akt Pathway, and Increase of Neurogenesis Are Associated with Therapeutic Improvement after Traumatic Brain Injury.
• Spreading depression.
• Statins increase neurogenesis in the dentate gyrus, reduce delayed neuronal death in the hippocampal CA3 region, and improve spatial learning in rat after traumatic brain injury.
• Subclinical photodynamic therapy treatment modifies the brain microenvironment and promotes glioma growth.
• The PI3K/Akt pathway mediates the neuroprotective effect of atorvastatin in extending thrombolytic therapy after embolic stroke in the rat.
• The Sonic hedgehog pathway mediates carbamylated erythropoietin-enhanced proliferation and differentiation of adult neural progenitor cells.
• The linkage of neural progenitor cell cycle profiles between embryonic and adult stroke models: Analytical approach II.
• Treatment of traumatic brain injury in rats with erythropoietin and carbamylated erythropoietin.