Neuroprotection of retinal ganglion cells in DBA/2J mice with GDNF-loaded biodegradable microspheres.

Neuroprotection of retinal ganglion cells in DBA/2J mice with GDNF-loaded biodegradable microspheres. Research Abstract Details 

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Neuroprotection of retinal ganglion cells in DBA/2J mice with GDNF-loaded biodegradable microspheres. Abstract Text:

M S Ward,A Khoobehi,E B Lavik,R Langer,M J Young,

This study aims to promote long-term retinal ganglion cell (RGC) survival in a spontaneous glaucoma model by injecting slow-release Poly(DL-lactide-co-glycolide) (PLGA) microspheres containing glial cell line-derived neurotrophic factor (GDNF) into the vitreous. Microspheres (1 microL) suspended in PBS were injected in ipsilateral eyes while contralateral eyes served as untreated controls. Mice were injected at 2 months intervals (1-4 injections) depending on the protocol. ELISA assay indicated a cumulative GDNF release of 35.4 ng/mg over 71 days. The release was nonlinear with an initial burst of over 50%. Mice displayed a 30% drop in RGC density by 8 months (p = 0.013) and 80% drop by 10 months (p < 0.01). GDNF delivery increased RGC survival in all groups. Mice receiving early treatment showed up to 3.5 times greater RGC density than untreated mice at 15 months survival (p < 0.05). No significant effect was found in sham or lens injury groups. Microsphere-delivered GDNF significantly increases long-term RGC survival in a spontaneous glaucoma model, although the nonlinear release kinetics suggest that burst release may play a role in this rescue. Neuroprotection with slow-release polymers with improved release kinetics should be further studied as a potential therapy for glaucoma and other diseases involving the loss of central nervous system neurons.

Neuroprotection of retinal ganglion cells in DBA/2J mice with GDNF-loaded biodegradable microspheres. Publishing Authors By Initials

MS Ward,A Khoobehi,EB Lavik,R Langer,MJ Young,

For similar vitreous body research abstracts see: vitreous body research

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Neuroprotection of retinal ganglion cells in DBA/2J mice with GDNF-loaded biodegradable microspheres. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of pharmaceutical sciences

VOLUME: 96

Page Numbers: 558-68

Journal Abbreviation:

ISSN: 0022-3549

DAY: 3

MONTH: Mar

YEAR: 2007

Neuroprotection of retinal ganglion cells in DBA/2J mice with GDNF-loaded biodegradable microspheres. Information

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LANGUAGE: eng

NlmUniqueID: 2985195

Neuroprotection of retinal ganglion cells in DBA/2J mice with GDNF-loaded biodegradable microspheres. Keywords Mesh Terms:

KEYWORDS: Vitreous Body

MESH TERMS: drug effects

Chemical & Substance for Abstract: Neuroprotection of retinal ganglion cells in DBA/2J mice with GDNF-loaded biodegradable microspheres. Information

Substance Name: Lactic Acid

Registry Number: 50-21-5

Grant and Affiliation Information for Neuroprotection of retinal ganglion cells in DBA/2J mice with GDNF-loaded biodegradable microspheres.

AFFILIATION: Schepens Eye Research Institute, Harvard Medical School, 20 Staniford Street, Boston, Massachusetts 02114, USA.

Country: United States

AGENCY: United States PHS

GRANT: NEI 09595

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MEDLINETA: J Pharm Sci

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