Neural progenitor cell transplantation and imaging in a large animal model.

Neural progenitor cell transplantation and imaging in a large animal model. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Neural progenitor cell transplantation and imaging in a large animal model. Abstract Text:

Lei Wang,Douglas R Martin,Henry J Baker,Kurt R Zinn,John C Kappes,Haitao Ding,Atoska S Gentry,Scarlett Harper,Evan Y Snyder,Nancy R Cox,Lei Wang,Douglas R Martin,Henry J Baker,Kurt R Zinn,John C Kappes,Haitao Ding,Atoska S Gentry,Scarlett Harper,Evan Y Snyder,Nancy R Cox,Lei Wang,Douglas R Martin,Henry J Baker,Kurt R Zinn,John C Kappes,Haitao Ding,Atoska S Gentry,Scarlett Harper,Evan Y Snyder,Nancy R Cox,

To evaluate neural stem/progenitor cell (NPC) transplantation therapy in cat models of neurodegenerative diseases, we have isolated, expanded and characterized feline NPCs (fNPCs) from normal fetal cat brain. Feline NPCs responsive to both human epidermal growth factor (hEGF) and human fibroblast growth factor 2 (hFGF2) proliferated as neurospheres, which were able to differentiate to neurons and glial cells. The analysis of growth factors indicated that both hEGF and hFGF2 were required for proliferation of fNPCs. In contrast to the effect on human NPCs, human leukemia inhibitory factor (hLIF) enhanced differentiation of fNPCs. Expanded fNPCs were injected into the brains of normal adult cats. Immunohistochemical analysis showed that the majority of transplanted cells were located adjacent to the injection site and some fNPCs differentiated into neurons. The survival of transplanted fNPCs over time was monitored using non-invasive bioluminescent imaging technology. This study provided the first evidence of allotransplantation of fNPCs into feline CNS. Cats have heterogeneous genetic backgrounds and possess neurological diseases that closely resemble analogous human diseases. The characterization of fNPCs and exploration of non-invasive bioluminescent imaging to track transplanted cells in this study will allow evaluation of NPC transplantation therapy using feline models of human neurological diseases.

Neural progenitor cell transplantation and imaging in a large animal model. Publishing Authors By Initials

L Wang,DR Martin,HJ Baker,KR Zinn,JC Kappes,H Ding,AS Gentry,S Harper,EY Snyder,NR Cox,L Wang,DR Martin,HJ Baker,KR Zinn,JC Kappes,H Ding,AS Gentry,S Harper,EY Snyder,NR Cox,L Wang,DR Martin,HJ Baker,KR Zinn,JC Kappes,H Ding,AS Gentry,S Harper,EY Snyder,NR Cox,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Neural progenitor cell transplantation and imaging in a large animal model. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Neuroscience research

VOLUME: 59

Page Numbers: 327-40

Journal Abbreviation: Neurosci. Res.

ISSN: 0168-0102

DAY: 23

MONTH: 08

YEAR: 2007

Neural progenitor cell transplantation and imaging in a large animal model. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8500749

Neural progenitor cell transplantation and imaging in a large animal model. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Neural progenitor cell transplantation and imaging in a large animal model. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Neural progenitor cell transplantation and imaging in a large animal model.

AFFILIATION: Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, Auburn, AL 36849-5525, United States.

Country: Ireland

AGENCY: United States NINDS

GRANT: NS57098

ACRONYM: NS

MEDLINETA: Neurosci Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Neural progenitor cell transplantation and imaging in a large animal model Related Publications

• Absence of metabotropic glutamate receptor-mediated plasticity in the neocortex of fragile X mice.
• Affinity purification of seminalplasmin and characterization of its interaction with calmodulin.
• Australian parasitological research: now and in 2000.
• CD4+ T cell-dependent reduction in hepatitis C virus-specific humoral immune responses after HIV infection.
• Cancer and the threat of death: the cognitive dynamics of death-thought suppression and its impact on behavioral health intentions.
• Chapter 7: Achievements and limitations of cervical cytology screening.
• Costs of alcohol and drug-involved crime.
• Determination of ciglitazone in dog plasma by reversed-phase high-performance liquid chromatography.
• Effects of adult aging and hearing loss on comprehension of rapid speech varying in syntactic complexity.
• European psychiatry: moving towards integration and harmony.
• Experimental apparatus for the study of photoabsorption processes of multiply charged ions by synchrotron radiation.
• Genetic studies of stuttering in a founder population.
• Hypertonic saline reverses stiffness in a Sprague-Dawley rat model of acute intestinal edema, leading to improved intestinal function.
• Immune effector mechanisms in parasitic infections.
• Measurement of intestinal edema using an impedance analyzer circuit.
• Metabotropic glutamate receptors differentially regulate GABAergic inhibition in thalamus.
• Microcalorimetric investigation of the interaction of calmodulin with seminalplasmin and myosin light chain kinase.
• Modulation of thalamic neuron excitability by orexins.
• News from the WPA Secretariat.
• Non-human primate fetal kidney transcriptome analysis indicates mammalian target of rapamycin (mTOR) is a central nutrient-responsive pathway.
• Only time can tell: unethical research and the passage of time.
• Organized unidirectional waves of ATP hydrolysis within a RecA filament.
• Prenatal methyl mercury exposure from fish consumption and child development: a review of evidence and perspectives from the Seychelles Child Development Study.
• Protection against cryptococcosis by using a murine gamma interferon-producing Cryptococcus neoformans strain.
• Role of the vitamin D receptor in hair follicle biology.
• Studies of pituitary-adrenal-testis interaction in sheep. II. The effects of repeated injections of adrenocorticotrophic hormone outside the breeding season.
• The 12th world congress of psychiatry.
• The 13th World Congress of Psychiatry, Cairo 2005: perspectives from the Secretariat.
• Use of retrovirus expression of interfering RNA to determine the contribution of activated k-ras and ras effector expression to human tumor cell growth.
• Using inhibitors of prenylation to block localization and transforming activity.