Neural induction in the absence of organizer in salamanders is mediated by MAPK.

Neural induction in the absence of organizer in salamanders is mediated by MAPK. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Neural induction in the absence of organizer in salamanders is mediated by MAPK. Abstract Text:

Cecilia Hurtado,E M De Robertis,Cecilia Hurtado,E M De Robertis,

Research on the mechanisms of embryonic induction had a great setback in the 1940s when Barth discovered and Holtfreter confirmed that ectoderm of Ambystoma maculatum salamander embryos could form brain tissue when cultured in a simple saline solution. We have revisited this classical experiment and found that when cultured animal cap ectoderm attaches to a glass substratum, it can self-organize to form complex organs such as brain vesicles, eyes, lens and olfactory placodes. Only anterior neural organs were generated. Under these culture conditions ERK became diphosphorylated, indicating a sustained activation of the Ras/MAPK pathway. Using sand particles as an example of a heterologous neural inducer similar results were obtained. Addition of U0126, a specific antagonist of MEK, the enzyme that phosphorylates ERK/MAPK, inhibited neural differentiation. The closely related control compound U0124 had no effect. We conclude that neural induction in the absence of organizer in A. maculatum requires Ras/MAPK-activation. These findings provide a molecular explanation for the activity of heterologous neural inducers that dominated thinking in amphibian experimental embryology for many decades.

Neural induction in the absence of organizer in salamanders is mediated by MAPK. Publishing Authors By Initials

C Hurtado,EM De Robertis,C Hurtado,EM De Robertis,

For similar embryonic structures: organizers, embryonic research abstracts see: embryonic structures: organizers, embryonic research

PUBMED ID PMID:

MEDLINE DATE:

Neural induction in the absence of organizer in salamanders is mediated by MAPK. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Developmental biology

VOLUME: 307

Page Numbers: 282-9

Journal Abbreviation: Dev. Biol.

ISSN: 0012-1606

DAY: 6

MONTH: 05

YEAR: 2007

Neural induction in the absence of organizer in salamanders is mediated by MAPK. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372762

Neural induction in the absence of organizer in salamanders is mediated by MAPK. Keywords Mesh Terms:

KEYWORDS: Organizers, Embryonic

MESH TERMS: embryology

Chemical & Substance for Abstract: Neural induction in the absence of organizer in salamanders is mediated by MAPK. Information

Substance Name: DNA Primers

Registry Number: 0

Grant and Affiliation Information for Neural induction in the absence of organizer in salamanders is mediated by MAPK.

AFFILIATION: Howard Hughes Medical Institute and Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, USA.

Country: United States

AGENCY: United States NICHD

GRANT: HD21502-20

ACRONYM: HD

MEDLINETA: Dev Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Neural induction in the absence of organizer in salamanders is mediated by MAPK Related Publications

• An electron microscope analysis of nerves infected with the B virus.
• Assay of thyrotropic hormone in human blood.
• Asymmetric mitosis: Unequal segregation of proteins destined for degradation.
• BMP-binding modules in chordin: a model for signalling regulation in the extracellular space.
• Crossveinless-2 Is a BMP feedback inhibitor that binds Chordin/BMP to regulate Xenopus embryonic patterning.
• Cytological and cytochemical bases of thyroid function.
• Endodermal Nodal-related signals and mesoderm induction in Xenopus.
• Integrating Patterning Signals: Wnt/GSK3 Regulates the Duration of the BMP/Smad1 Signal.
• Mouse Crossveinless-2 is the vertebrate homolog of a Drosophila extracellular regulator of BMP signaling.
• Neural induction in the absence of organizer in salamanders is mediated by MAPK.
• Postnatal evolution of steroidogenic cells in the chick ovary.
• Proteolytic activity in the physiology, pathology and therapeutics of the thyroid gland.
• The Xenopus homologue of Bicaudal-C is a localized maternal mRNA that can induce endoderm formation.
• The evolutionarily conserved BMP-binding protein Twisted gastrulation promotes BMP signalling.
• The head inducer Cerberus is a multifunctional antagonist of Nodal, BMP and Wnt signals.
• The opposing homeobox genes Goosecoid and Vent1/2 self-regulate Xenopus patterning.
• Zebrafish paraxial protocadherin is a downstream target of spadetail involved in morphogenesis of gastrula mesoderm.
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [The head inducer Cerberus in a multivalent extracellular inhibitor]