Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli.

Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli. Research Abstract Details 

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Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli. Abstract Text:

Umechiyo Tokumoto,Shinobu Nomura,Yoshiko Minami,Hisaaki Mihara,Shin-Ichiro Kato,Tatsuo Kurihara,Nobuyoshi Esaki,Hiroshi Kanazawa,Hiroshi Matsubara,Yasuhiro Takahashi,

The assembly of iron-sulfur (Fe-S) clusters is mediated by complex machinery which, in Escherichia coli, is encoded by the iscRSUA-hscBA-fdx-ORF3 gene cluster. Here, we demonstrate the network of protein-protein interactions among the components involved in the machinery. We have constructed (His)(6)-tagged versions of the components and identified their interacting partners that were co-purified from E. coli extracts with a Ni-affinity column. Direct associations of the defined pair of proteins were further examined in yeast cells using the two-hybrid system. In accord with the previous in vitro binding and kinetic experiments, interactions were observed for the combinations of IscS and IscU, IscU and HscB, IscU and HscA, and HscB and HscA. In addition, we have identified previously unreported interactions between IscS and Fdx, IscS and ORF3, IscA and HscA, and HscA and Fdx. We also found, by site-directed mutational analysis combined with the two-hybrid system, that two cysteine residues in IscU are essential for binding with HscB but not with IscS. Despite the complex network of interactions in various combinations of components, heteromultimeric complexes were not observed in our experiments except for the putative oligomeric form of IscU-IscS-ORF3. Thus, the sequential association and dissociation among the IscS, IscU, IscA, HscB, HscA, Fdx, and ORF3 proteins may be a critical process in the assembly of Fe-S clusters.

Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli. Publishing Authors By Initials

U Tokumoto,S Nomura,Y Minami,H Mihara,S Kato,T Kurihara,N Esaki,H Kanazawa,H Matsubara,Y Takahashi,

For similar enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: galactosidases: beta-galactosidase research abstracts see: enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: galactosidases: beta-galactosidase research

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Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 131

Page Numbers: 713-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 2002

Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli. Keywords Mesh Terms:

KEYWORDS: beta-Galactosidase

MESH TERMS: metabolism

Chemical & Substance for Abstract: Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli. Information

Substance Name: beta-Galactosidase

Registry Number: EC 3.2.1.23

Grant and Affiliation Information for Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli.

AFFILIATION: Department of Biology, Graduate School of Science, Osaka University, Machikaneyama, Toyonaka, Osaka 560-0043, Japan.

Country: Japan

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MEDLINETA: J Biochem

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