Nerve growth factor-immobilized polypyrrole: bioactive electrically conducting polymer for enhanced neurite extension.

Nerve growth factor-immobilized polypyrrole: bioactive electrically conducting polymer for enhanced neurite extension. Research Abstract Details 

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Nerve growth factor-immobilized polypyrrole: bioactive electrically conducting polymer for enhanced neurite extension. Abstract Text:

Natalia Gomez,Christine E Schmidt,

Biomaterials that present multiple stimuli are attractive for a number of biomedical applications. In particular, electrical and biological cues are important factors to include in interfaces with neurons for applications such as nerve conduits and neural probes. Here, we report the combination of these two stimuli, by immobilizing nerve growth factor (NGF) on the surface of the electrically conducting polymer polypyrrole (PPy). NGF was immobilized using an intermediate linker provided by a layer of polyallylamine conjugated to an arylazido functional group. Upon exposure to UV light and activation of the azido groups, NGF was fixed to the substrate. Three different surface concentrations were obtained (0.21-0.98 ng/mm(2)) and similar levels of neurite extension were observed on immobilized NGF as with soluble NGF. Additionally, electrical stimulation experiments were conducted with the modified polymer and revealed a 50% increase in neurite outgrowth in PC12 cells compared to experiments without electrical stimulation. This novel modification of PPy provides both electrical and biological stimulation, by presenting tethered growth factors and only producing a small decrease in the material's properties (conductivity approximately 10 S cm(-1)) when compared to other modification techniques (conductivity approximately 10(-3)-10(-6) S cm(-1)).

Nerve growth factor-immobilized polypyrrole: bioactive electrically conducting polymer for enhanced neurite extension. Publishing Authors By Initials

N Gomez,CE Schmidt,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research

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MEDLINE DATE:

Nerve growth factor-immobilized polypyrrole: bioactive electrically conducting polymer for enhanced neurite extension. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of biomedical materials research. Part A

VOLUME: 81

Page Numbers: 135-49

Journal Abbreviation:

ISSN: 1549-3296

DAY: 3

MONTH: Apr

YEAR: 2007

Nerve growth factor-immobilized polypyrrole: bioactive electrically conducting polymer for enhanced neurite extension. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101234237

Nerve growth factor-immobilized polypyrrole: bioactive electrically conducting polymer for enhanced neurite extension. Keywords Mesh Terms:

KEYWORDS: Rats

MESH TERMS: chemistry

Chemical & Substance for Abstract: Nerve growth factor-immobilized polypyrrole: bioactive electrically conducting polymer for enhanced neurite extension. Information

Substance Name: Nerve Growth Factor

Registry Number: 9061-61-4

Grant and Affiliation Information for Nerve growth factor-immobilized polypyrrole: bioactive electrically conducting polymer for enhanced neurite extension.

AFFILIATION: Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712-1062, USA.

Country: United States

AGENCY: United States NIBIB

GRANT: R01 EB 004529

ACRONYM: EB

MEDLINETA: J Biomed Mater Res A

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