Nerve growth factor expression after plantar incision in the rat.

Nerve growth factor expression after plantar incision in the rat. Research Abstract Details 

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Nerve growth factor expression after plantar incision in the rat. Abstract Text:

Chaoran Wu,Leila Boustany,Hong Liang,Timothy J Brennan,

BACKGROUND: Postoperative pain control remains a significant problem. Advances will proceed if we can further reveal the underlying mechanisms of incisional pain and its mediators. Previous studies have demonstrated that nerve growth factor (NGF) is released in incised tissue and contributes to hyperalgesia in incisional pain. The purpose of this study is to examine the expression of NGF in skin after planter incision. METHODS: Adult Sprague-Dawley rats underwent incision at the plantar aspect of hind paw. The NGF messenger RNA (mRNA) was measured at various times after incision by polymerase chain reaction. NGF protein expression was detected by Western blot and immunohistochemistry in incisions. RESULTS: NGF mRNA increased from 2 to 4 h after incision and was the same as control by postoperative day 1. A large-molecular-weight form of NGF, approximately 75 kd, was found in normal skin. The large-molecular-weight NGF protein increased 4 h after incision and returned to baseline on postoperative day 7. The skin immediately adjacent to the incision had the greatest NGF expression. Immunohistochemical staining for NGF was present adjacent to the incision and localized in Schwann cells and axons. CONCLUSION: NGF mRNA is increased and a large-molecular-weight form of NGF protein is expressed in the region adjacent to the incision. NGF immunoreactivity is present in nerve bundles; both Schwann cells and axons are labeled. Immunoreactive NGF in axons is likely taken up into cut axons. This study suggests some common mechanisms for neuropathic and incisional pain.

Nerve growth factor expression after plantar incision in the rat. Publishing Authors By Initials

C Wu,L Boustany,H Liang,TJ Brennan,

For similar enzymes and coenzymes: enzymes: hydrolases: esterases: thiolester hydrolases: ubiquitin thiolesterase research abstracts see: enzymes and coenzymes: enzymes: hydrolases: esterases: thiolester hydrolases: ubiquitin thiolesterase research

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Nerve growth factor expression after plantar incision in the rat. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Anesthesiology

VOLUME: 107

Page Numbers: 128-35

Journal Abbreviation: Anesthesiology

ISSN: 0003-3022

DAY: 3

MONTH: Jul

YEAR: 2007

Nerve growth factor expression after plantar incision in the rat. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 1300217

Nerve growth factor expression after plantar incision in the rat. Keywords Mesh Terms:

KEYWORDS: Ubiquitin Thiolesterase

MESH TERMS: metabolism

Chemical & Substance for Abstract: Nerve growth factor expression after plantar incision in the rat. Information

Substance Name: Ubiquitin Thiolesterase

Registry Number: EC 3.1.2.15

Grant and Affiliation Information for Nerve growth factor expression after plantar incision in the rat.

AFFILIATION: Department of Anesthesia, University of Iowa Hospitals and Clinics, Iowa City, Iowa 52242, USA. chaoran-wu@uiowa.edu

Country: United States

AGENCY: United States NIGMS

GRANT: GM-55831

ACRONYM: GM

MEDLINETA: Anesthesiology

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