Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia.

Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia. Abstract Text:

Sheila Rydziel,Lisa Stadmeyer,Stefano Zanotti,Deena Durant,Anna Smerdel-Ramoya,Ernesto Canalis,

Nephroblastoma overexpressed (Nov), a member of the Cyr 61, connective tissue growth factor, Nov (CCN) family of proteins, is expressed by osteoblasts, but its function in cells of the osteoblastic lineage is not known. We investigated the effects of Nov overexpression by transducing murine ST-2 stromal and MC3T3 osteoblastic cells with a retroviral vector where Nov is under the control of the cytomegalovirus promoter. We also examined the skeletal phenotype of transgenic mice expressing Nov under the control of the human osteocalcin promoter. Overexpression of Nov in ST-2 cells inhibited the appearance of mineralized nodules and decreased alkaline phosphatase activity and osteocalcin mRNA levels. Nov overexpression inhibited the effect of bone morphogenetic protein (BMP)-2 on the phosphorylation of Smad 1/5/8; on the transactivation of 12xSBE-Oc-pGL3, a BMP/Smad signaling reporter construct, and of Wnt 3 on cytoplasmic beta-catenin levels; and on the transactivation of the Wnt/beta-catenin signaling reporter construct 16xTCF-Luc. Nov overexpression did not activate Notch or transforming growth factor beta signaling. Glutathione S-transferase pulldown assays demonstrated direct Nov-BMP interactions. Nov transgenic mice exhibited osteopenia. In conclusion, Nov binds BMP-2 and antagonizes BMP-2 and Wnt activity, and its overexpression inhibits osteoblastogenesis and causes osteopenia.

Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia. Publishing Authors By Initials

S Rydziel,L Stadmeyer,S Zanotti,D Durant,A Smerdel-Ramoya,E Canalis,

For similar peptides: intercellular signaling peptides and proteins: wnt proteins research abstracts see: peptides: intercellular signaling peptides and proteins: wnt proteins research

PUBMED ID PMID:

MEDLINE DATE:

Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 19762-72

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 10

MONTH: 05

YEAR: 2007

Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia. Keywords Mesh Terms:

KEYWORDS: Wnt Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia.

AFFILIATION: Department of Research, Saint Francis Hospital and Medical Center, Hartford, Connecticut 06105, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK45227

ACRONYM: DK

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Nephroblastoma overexpressed Nov inhibits osteoblastogenesis and causes osteopenia Related Publications

• Activation of the ERK pathway in osteoblastic cells, role of gremlin and BMP-2.
• Bone morphogenetic protein-2 suppresses collagenase-3 promoter activity in osteoblasts through a runt domain factor 2 binding site.
• Bone morphogenetic proteins induce gremlin, a protein that limits their activity in osteoblasts.
• CCAAT/Enhancer-binding protein homologous protein (CHOP) decreases bone formation and causes osteopenia.
• CCAAT/enhancer binding protein homologous protein (DDIT3) induces osteoblastic cell differentiation.
• Conditional deletion of gremlin causes a transient increase in bone formation and bone mass.
• Connective tissue growth factor enhances osteoblastogenesis in vitro.
• Effect of growth factors on bone cell replication and differentiation.
• Effects of cortisol and bone morphogenetic protein-2 on stromal cell differentiation: correlation with CCAAT-enhancer binding protein expression.
• Expression and regulation of CCN genes in murine osteoblasts.
• Gene delivery by retroviruses.
• Growth hormone, insulin-like growth factors, and the skeleton.
• Local bone growth factors.
• Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia.
• Nephroblastoma overexpressed (nov) is a novel bone morphogenetic protein antagonist.
• Notch inhibits osteoblast differentiation and causes osteopenia.
• Notch signaling in osteoblasts.
• Regulation of collagenase-3 by bone morphogenetic protein-2 in bone cell cultures.
• Skeletal overexpression of connective tissue growth factor impairs bone formation and causes osteopenia.
• Transcriptional regulation of connective tissue growth factor by cortisol in osteoblasts.
• Transient focal lesion in the splenium of the corpus callosum: MR imaging with an attempt to clinical-physiopathological explanation and review of the literature.
• Twisted gastrulation, a bone morphogenetic protein agonist/antagonist, is not required for post-natal skeletal function.