Neovascularization effect of biodegradable gelatin microspheres incorporating basic fibroblast growth factor.

Neovascularization effect of biodegradable gelatin microspheres incorporating basic fibroblast growth factor. Research Abstract Details 

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Neovascularization effect of biodegradable gelatin microspheres incorporating basic fibroblast growth factor. Abstract Text:

Y Tabata,S Hijikata,M Muniruzzaman,Y Ikada,

Biodegradable microspheres were prepared through glutaraldehyde cross-linking of gelatin without using any surfactants as a carrier matrix of basic fibroblast growth factor (bFGF). In the in vitro system, bFGF was sorbed to microspheres of acidic gelatin with an isoelectric point (IEP) of 5.0, but not to those of basic gelatin with an IEP of 9.0. The rate of bFGF sorption to the acidic gelatin microsphere in phosphate-buffered saline solution (pH 7.4) was smaller than that in water. Following incorporation of bFGF into the microspheres at 4 degrees C for 12 h, bFGF release from the bFGF-incorporating microspheres was studied. Approximately 30% of incorporated bFGF was released from the acidic gelatin microsphere within the initial 3 h, followed by no substantial release, whereas the basic gelatin microsphere released almost completely the incorporated bFGF within 1 day. It is likely that when basic bFGF molecules were immobilized to the acidic gelatin constituting microspheres through polyion complexation, they were not readily released under the in vitro nondegradation condition of gelatin. Incorporation of anionic carboxylmethyl cellulose (CMC) into the acidic gelatin microspheres reduced the amount of bFGF desorbed initially. This indicates that the initial burst is ascribed to free bFGF which is not ionically interacted with the acidic gelatin. CMC will function as a bFGF sorbent to suppress the initial leakage from the microspheres. When injected subcutaneously into the mouse back, bFGF-incorporating acidic gelatin microspheres were degraded over time and induced neovascularization around the injection site, in marked contrast to bFGF in the solution form. CMC incorporation slowed down the biodegradation and vascularization effect of bFGF-incorporating gelatin microspheres. It was concluded that the gelatin microsphere was a promising carrier matrix of bFGF to enhance the vascularization effect.

Neovascularization effect of biodegradable gelatin microspheres incorporating basic fibroblast growth factor. Publishing Authors By Initials

Y Tabata,S Hijikata,M Muniruzzaman,Y Ikada,

For similar integumentary system: skin research abstracts see: integumentary system: skin research

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Neovascularization effect of biodegradable gelatin microspheres incorporating basic fibroblast growth factor. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 10

Page Numbers: 79-94

Journal Abbreviation:

ISSN: 0920-5063

DAY: 20

MONTH: 02

YEAR: 1999

Neovascularization effect of biodegradable gelatin microspheres incorporating basic fibroblast growth factor. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9007393

Neovascularization effect of biodegradable gelatin microspheres incorporating basic fibroblast growth factor. Keywords Mesh Terms:

KEYWORDS: Skin

MESH TERMS: blood supply

Chemical & Substance for Abstract: Neovascularization effect of biodegradable gelatin microspheres incorporating basic fibroblast growth factor. Information

Substance Name: Carboxymethylcellulose

Registry Number: 9004-32-4

Grant and Affiliation Information for Neovascularization effect of biodegradable gelatin microspheres incorporating basic fibroblast growth factor.

AFFILIATION: Research Center for Biomedical Engineering, Kyoto University, Japan.

Country: NETHERLANDS

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MEDLINETA: J Biomater Sci Polym Ed

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