[Neomycin inhibits nicotinic receptor-mediated response in pheochromocytoma (PC12) cells]

[Neomycin inhibits nicotinic receptor-mediated response in pheochromocytoma (PC12) cells] Research Abstract Details 

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[Neomycin inhibits nicotinic receptor-mediated response in pheochromocytoma (PC12) cells] Abstract Text:

Li-Jun Shi,Chun-An Wang,

Neomycin is one of the aminoglycoside antibiotics, and on the cellular level it inhibits phospholipase C. The effects of neomycin on the acetylcholine (ACh)-induced current (I(ACh)) were studied in pheochromocytoma (PC12) cells by using the whole-cell clamp technique. The I(ACh) on PC12 cells proved to be generated through activation of the neuronal nicotinic receptor. ACh (30 micromol/L) induced an inward current at a holding potential of -80 mV. When the cells were applied with neomycin (0.01~1 mmol/L) and ACh (30 micromol/L) simultaneously, an inhibitory effect of neomycin on the peak of I(ACh) was observed. This effect was fast, reversible and concentration-dependent. Pretreatment with neomycin for 3~8 min had no influence on its inhibitory effect. Activation of protein kinase C by using an exogenous activator exerted an inhibitory action on I(ACh). However, intracellular dialysis with a PKC inhibitor (PKCI 19-31, 0.1~5 micromol/L) did not affect the inhibitory effect of neomycin. The results obtained suggest that neomycin exerts an inhibitory effect on I(ACh) without involvement of the blockage of phospholipase C.

[Neomycin inhibits nicotinic receptor-mediated response in pheochromocytoma (PC12) cells] Publishing Authors By Initials

LJ Shi,CA Wang,

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[Neomycin inhibits nicotinic receptor-mediated response in pheochromocytoma (PC12) cells] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Sheng li xue bao : [Acta physiologica Sinica]

VOLUME: 54

Page Numbers: 441-5

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ISSN: 0371-0874

DAY: 25

MONTH: Oct

YEAR: 2002

[Neomycin inhibits nicotinic receptor-mediated response in pheochromocytoma (PC12) cells] Information

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LANGUAGE: chi

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AFFILIATION: Department of Physiology, Beijing Medical College of PLA, Beijing, China. l_j_shi@hotmail.com

Country: China

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MEDLINETA: Sheng Li Xue Bao

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