Nebulin Interacts with CapZ and Regulates Thin Filament Architecture within the Z-Disc.

Nebulin Interacts with CapZ and Regulates Thin Filament Architecture within the Z-Disc. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Nebulin Interacts with CapZ and Regulates Thin Filament Architecture within the Z-Disc. Abstract Text:

The barbed ends of actin filaments in striated muscle are anchored within the Z-disc and capped by CapZ; this protein blocks actin polymerization and depolymerization in vitro. The mature lengths of the thin filaments are likely specified by the giant "molecular ruler" nebulin, which spans the length of the thin filament. Here, we report that CapZ specifically interacts with the C terminus of nebulin (modules 160-164) in blot overlay, solid-phase binding, tryptophan fluorescence, and SPOTs membrane assays. Binding of nebulin modules 160-164 to CapZ does not affect the ability of CapZ to cap actin filaments in vitro, consistent with our observation that neither of the two C-terminal actin binding regions of CapZ is necessary for its interaction with nebulin. Knockdown of nebulin in chick skeletal myotubes using small interfering RNA results in a reduction of assembled CapZ, and, strikingly, a loss of the uniform alignment of the barbed ends of the actin filaments. These data suggest that nebulin restricts the position of thin filament barbed ends to the Z-disc via a direct interaction with CapZ. We propose a novel molecular model of Z-disc architecture in which nebulin interacts with CapZ from a thin filament of an adjacent sarcomere, thus providing a structural link between sarcomeres.

Nebulin Interacts with CapZ and Regulates Thin Filament Architecture within the Z-Disc. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Nebulin Interacts with CapZ and Regulates Thin Filament Architecture within the Z-Disc. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular biology of the cell

VOLUME: 19

Page Numbers: 1837-47

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 13

MONTH: 02

YEAR: 2008

Nebulin Interacts with CapZ and Regulates Thin Filament Architecture within the Z-Disc. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9201390

Nebulin Interacts with CapZ and Regulates Thin Filament Architecture within the Z-Disc. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Nebulin Interacts with CapZ and Regulates Thin Filament Architecture within the Z-Disc. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Nebulin Interacts with CapZ and Regulates Thin Filament Architecture within the Z-Disc.

AFFILIATION: Departments of Cell Biology and Anatomy and *Molecular and Cellular Biology, The University of Arizona, Tucson, AZ 85721-0106.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mol Biol Cell

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Nebulin Interacts with CapZ and Regulates Thin Filament Architecture within the Z-Disc Related Publications

• A spatial scan statistic for survival data.
• Application of the Kushida morphometric model in patients with sleep-disordered breathing.
• Assessment of urinary mutagens presence in a population of non smokers.
• Cervical cancer screening in the Ferrara Local Health Authority catchment area.
• Deviated nose correction by using the spreader graft in the convex side.
• Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae).
• Intrahisian conduction disease and junctional ectopic tachycardia.
• Intraoperative awareness--a three year prospective study using bispectral index monitor (BIS).
• Is surgery a risk factor for Creutzfeldt-Jakob disease? Outcome variation by control choice and exposure assessments.
• Isolated left cor triatriatum: a rare cause of effort dyspnoea in the adult.
• Lasp-2 expression, localization, and ligand interactions: A new Z-disc scaffolding protein.
• Nebulin Interacts with CapZ and Regulates Thin Filament Architecture within the Z-Disc.
• Rhinolithiasis as cause of oronasal fistula.
• Spherites in the midgut epithelial cells of the sugarcane borer parasitized by Cotesia flavipes.
• Temporal relationship between successful pancreas transplantation and control of ocular complications in alloxan-induced diabetic rats.
• Ultrastructure of Giardia canis trophozoites.
• Ultrastructure of the excretory duct in the silk gland of the sugarcane borer Diatraea saccharalis (Lepidoptera: Pyralidae).
• [Inverted papilloma in the sphenoethmoidal recess.]
• [Psychological aspect in the treatment of the partially edentulous]
• [Ultrastructural evaluation of mitomycin C toxic effects on the ciliary epithelium of normal rabbits eyes with and without aqueous humor suppressant treatment]