Near infrared dyes as lifetime solvatochromic probes for micropolarity measurements of biological systems.

Near infrared dyes as lifetime solvatochromic probes for micropolarity measurements of biological systems. Research Abstract Details 

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Near infrared dyes as lifetime solvatochromic probes for micropolarity measurements of biological systems. Abstract Text:

Mikhail Y Berezin,Hyeran Lee,Walter Akers,Samuel Achilefu,Mikhail Y Berezin,Hyeran Lee,Walter Akers,Samuel Achilefu,

The polarity of biological mediums controls a host of physiological processes such as digestion, signaling, transportation, metabolism, and excretion. With the recent widespread use of near-infrared (NIR) fluorescent dyes for biological imaging of cells and living organisms, reporting medium polarity with these dyes would provide invaluable functional information in addition to conventional optical imaging parameters. Here, we report a new approach to determine polarities of macro- and microsystems for in vitro and potential in vivo applications using NIR polymethine molecular probes. Unlike the poor solvatochromic response of NIR dyes in solvents with diverse polarity, their fluorescence lifetimes are highly sensitive, increasing by a factor of up to 8 on moving from polar to nonpolar mediums. We also established a correlation between fluorescence lifetime and solvent orientation polarizability and developed a lifetime polarity index for determining the polarity of complex systems, including micelles and albumin binding sites. Because of the importance of medium polarity in molecular, cellular, and biochemical processes and the significance of reduced autofluorescence and deep tissue penetration of light in the NIR region, the findings reported herein represent an important advance toward using NIR molecular probes to measure the polarity of complex biological systems in vitro and in vivo.

Near infrared dyes as lifetime solvatochromic probes for micropolarity measurements of biological systems. Publishing Authors By Initials

MY Berezin,H Lee,W Akers,S Achilefu,MY Berezin,H Lee,W Akers,S Achilefu,

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Near infrared dyes as lifetime solvatochromic probes for micropolarity measurements of biological systems. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biophysical journal

VOLUME: 93

Page Numbers: 2892-9

Journal Abbreviation: Biophys. J.

ISSN: 0006-3495

DAY: 15

MONTH: 06

YEAR: 2007

Near infrared dyes as lifetime solvatochromic probes for micropolarity measurements of biological systems. Information

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LANGUAGE: eng

NlmUniqueID: 370626

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Grant and Affiliation Information for Near infrared dyes as lifetime solvatochromic probes for micropolarity measurements of biological systems.

AFFILIATION: Department of Radiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

Country: United States

AGENCY: United States NCI

GRANT: U54 CA119342

ACRONYM: CA

MEDLINETA: Biophys J

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