Natural combinatorial peptide libraries in cyanobacterial symbionts of marine ascidians.

Natural combinatorial peptide libraries in cyanobacterial symbionts of marine ascidians. Research Abstract Details 

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Natural combinatorial peptide libraries in cyanobacterial symbionts of marine ascidians. Abstract Text:

A large family of cytotoxic cyclic peptides exemplified by the patellamides has been isolated from ascidians harboring the obligate cyanobacterial symbionts Prochloron spp.. Genome sequence analysis of these symbionts has revealed that Prochloron spp. synthesize patellamides by a ribosomal pathway. To understand how this pathway evolved to produce a suite of related metabolites, we analyzed 46 prochloron-containing ascidians from the tropical Pacific Ocean for the presence of patellamide biosynthetic genes and taxonomic markers. Here, we show that Prochloron spp. generate a diverse library of patellamides using small, hypervariable cassettes within a conserved genetic background. Each symbiont strain contains a single pathway, and mixtures of symbionts within ascidians lead to the accumulation of chemical libraries. We used this information to engineer the production of a new cyclic peptide in Escherichia coli, thereby demonstrating the power of comparative analysis of closely related symbiotic pathways to direct the genetic synthesis of new molecules.

Natural combinatorial peptide libraries in cyanobacterial symbionts of marine ascidians. Publishing Authors By Initials

For similar animals: chordata: chordata, nonvertebrate: urochordata research abstracts see: animals: chordata: chordata, nonvertebrate: urochordata research

PUBMED ID PMID:

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Natural combinatorial peptide libraries in cyanobacterial symbionts of marine ascidians. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Nature chemical biology

VOLUME: 2

Page Numbers: 729-35

Journal Abbreviation: Nat. Chem. Biol.

ISSN: 1552-4450

DAY: 5

MONTH: 11

YEAR: 2006

Natural combinatorial peptide libraries in cyanobacterial symbionts of marine ascidians. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101231976

Natural combinatorial peptide libraries in cyanobacterial symbionts of marine ascidians. Keywords Mesh Terms:

KEYWORDS: Urochordata

MESH TERMS: microbiology

Chemical & Substance for Abstract: Natural combinatorial peptide libraries in cyanobacterial symbionts of marine ascidians. Information

Substance Name: Peptide Library

Registry Number: 0

Grant and Affiliation Information for Natural combinatorial peptide libraries in cyanobacterial symbionts of marine ascidians.

AFFILIATION:

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM 071425-01A1

ACRONYM: GM

MEDLINETA: Nat Chem Biol

REFSOURCE: Nat Chem Biol. 2006 Dec;2(12):661-2

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