Nanocrystal quantum dot-conjugated anti-myeloperoxidase antibody as the detector of activated neutrophils.

Nanocrystal quantum dot-conjugated anti-myeloperoxidase antibody as the detector of activated neutrophils. Research Abstract Details 

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Nanocrystal quantum dot-conjugated anti-myeloperoxidase antibody as the detector of activated neutrophils. Abstract Text:

Akiyoshi Hoshino,Tomokazu Nagao,Akira Nakasuga,Akiko Ishida-Okawara,Kazuo Suzuki,Masato Yasuhara,Kenji Yamamoto,Akiyoshi Hoshino,Tomokazu Nagao,Akira Nakasuga,Akiko Ishida-Okawara,Kazuo Suzuki,Masato Yasuhara,Kenji Yamamoto,

Fluorescent nanocrystal quantum dots (QDs) have been applied to a wide range of biological studies by taking advantage of their fluorescence properties. Here we show that QDs conjugated with antibody against neutrophil peroxidase, myeloperoxidase (MPO). We designed a novel method to conjugate QDs to antibody without losing any antibody function including their antigen recognizing and Fc-receptor binding activities. When we applied anti-MPO antibody (Ab) with conventional organic probes in the case of immunostaining of living cells, the antibodies lost their fluorescence because of MPO enzymic activity to produce reactive oxygen species. Our QD-conjugated anti-MPO (alpha-MPO-QDs) can detect MPO on the surface of activated neutrophils. In addition, anti-MPO-QDs did not react to the inactivated neutrophils. In conclusion, we demonstrated that antibody visualized the expression of MPO on the neutrophil surface after stimulation with proinflammatory cytokines. Taken together, these techniques have the possibility that QDs can reveal the activation of neutrophils by immunostaining and flow cytometric analysis as a powerful tool for diagnosis of the neutrophil activation in vitro.

Nanocrystal quantum dot-conjugated anti-myeloperoxidase antibody as the detector of activated neutrophils. Publishing Authors By Initials

A Hoshino,T Nagao,A Nakasuga,A Ishida-Okawara,K Suzuki,M Yasuhara,K Yamamoto,A Hoshino,T Nagao,A Nakasuga,A Ishida-Okawara,K Suzuki,M Yasuhara,K Yamamoto,

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Nanocrystal quantum dot-conjugated anti-myeloperoxidase antibody as the detector of activated neutrophils. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: IEEE transactions on nanobioscience

VOLUME: 6

Page Numbers: 341-5

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ISSN: 1536-1241

DAY: 25

MONTH: Dec

YEAR: 2007

Nanocrystal quantum dot-conjugated anti-myeloperoxidase antibody as the detector of activated neutrophils. Information

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LANGUAGE: eng

NlmUniqueID: 101152869

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Grant and Affiliation Information for Nanocrystal quantum dot-conjugated anti-myeloperoxidase antibody as the detector of activated neutrophils.

AFFILIATION: Research Institute, International Medical Center of Japan, Shinjuku-ku, Tokyo 162-8655, Japan.

Country: United States

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MEDLINETA: IEEE Trans Nanobioscience

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