Na(+)-independent choline transport in rat retinal capillary endothelial cells.

Na(+)-independent choline transport in rat retinal capillary endothelial cells. Research Abstract Details 

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Na(+)-independent choline transport in rat retinal capillary endothelial cells. Abstract Text:

Masatoshi Tomi,Kanako Arai,Masanori Tachikawa,Ken-ichi Hosoya,Masatoshi Tomi,Kanako Arai,Masanori Tachikawa,Ken-ichi Hosoya,

The purpose of this study was to clarify the mechanism of the inner blood-retinal barrier (inner BRB) transport of choline and examine the choline uptake ability of rat choline transporter-like protein (CTL) 1. The transcript level of CTL1 in a conditionally immortalized rat retinal capillary endothelial cell line (TR-iBRB2) is more than 100-fold greater than that of CTL3 and CTL4, and no expression of organic cation transporter (OCT) mRNA was detected. The apparent influx permeability clearance of [(3)H]choline in the rat retina was found to be 271 microl/(min x g retina). The [(3)H]choline uptake by TR-iBRB2 cells was Na(+)-independent, potential-dependent, and concentration-dependent with Michaelis-Menten constants of 6.44 microM and 99.7 microM, and inhibited by several organic cations but not tetraethylammonium. The inhibition of CTL1 mRNA by small interfering RNA had little effect on the [(3)H]choline uptake by TR-iBRB2 cells. Rat CTL1-expressing Xenopus laevis oocytes exhibited an increase in the [(3)H]choline uptake by 45% compared with a control. In conclusion, our findings are consistent with Na(+)-independent choline transport being the mechanism for blood-to-retina transport of choline at the inner BRB. Although rat CTL1 expression is associated with the choline uptake, CTL1 does not play a major role in the choline uptake at the inner BRB.

Na(+)-independent choline transport in rat retinal capillary endothelial cells. Publishing Authors By Initials

M Tomi,K Arai,M Tachikawa,K Hosoya,M Tomi,K Arai,M Tachikawa,K Hosoya,

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Na(+)-independent choline transport in rat retinal capillary endothelial cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Neurochemical research

VOLUME: 32

Page Numbers: 1833-42

Journal Abbreviation: Neurochem. Res.

ISSN: 0364-3190

DAY: 23

MONTH: 05

YEAR: 2007

Na(+)-independent choline transport in rat retinal capillary endothelial cells. Information

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LANGUAGE: eng

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AFFILIATION: Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

Country: United States

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MEDLINETA: Neurochem Res

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