Na channel expression and activity in the medullary collecting duct of rat kidney.

Na channel expression and activity in the medullary collecting duct of rat kidney. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Na channel expression and activity in the medullary collecting duct of rat kidney. Abstract Text:

Gustavo Frindt,Zuhal Ergonul,Lawrence G Palmer,

The expression and activity of epithelial Na(+) channels (ENaC) in the medullary collecting duct of the rat kidney were examined using a combination of whole cell patch-clamp measurements of amiloride-sensitive currents (I(Na)) in split-open tubules and Western blot analysis of alpha-, beta-, and gamma-ENaC proteins. In the outer medullary collecting duct, amiloride-sensitive currents were undetectable in principal cells from control animals but were robust when rats were treated with aldosterone (I(Na) = 960 +/- 160 pA/cell) or fed a low-Na diet (I(Na) = 440 +/- 120 pA/cell). In both cases, the currents were similar to those measured in principal cells of the cortical collecting duct from the same animals. In the inner medullary collecting duct, currents were much lower, averaging 120 +/- 20 pA/cell in aldosterone-treated rats. Immunoblots showed that all three ENaC subunits were expressed in the cortex, outer medulla, and inner medulla of the rat kidney. When rats were fed a low-Na diet for 1 wk, similar changes in alpha- and gamma-ENaC occurred in all three regions of the kidney; the amounts of full-length as well as putative cleaved alpha-ENaC protein increased, and the fraction of gamma-ENaC protein in the cleaved state increased at the expense of the full-length protein. The appearance of a presumably fully glycosylated form of beta-ENaC in Na-depleted animals was observed mainly in the outer and inner medulla. These findings suggest that the capability of hormone-regulated, channel-mediated Na reabsorption by the nephron extends at least into the outer medullary collecting duct.

Na channel expression and activity in the medullary collecting duct of rat kidney. Publishing Authors By Initials

G Frindt,Z Ergonul,LG Palmer,

For similar inorganic chemicals: sodium compounds: sodium, dietary research abstracts see: inorganic chemicals: sodium compounds: sodium, dietary research

PUBMED ID PMID:

MEDLINE DATE:

Na channel expression and activity in the medullary collecting duct of rat kidney. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Renal physiology

VOLUME: 292

Page Numbers: F1190-6

Journal Abbreviation:

ISSN: 0363-6127

DAY: 2

MONTH: 01

YEAR: 2007

Na channel expression and activity in the medullary collecting duct of rat kidney. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901990

Na channel expression and activity in the medullary collecting duct of rat kidney. Keywords Mesh Terms:

KEYWORDS: Sodium, Dietary

MESH TERMS: administration & dosage

Chemical & Substance for Abstract: Na channel expression and activity in the medullary collecting duct of rat kidney. Information

Substance Name: Aldosterone

Registry Number: 52-39-1

Grant and Affiliation Information for Na channel expression and activity in the medullary collecting duct of rat kidney.

AFFILIATION: Dept. of Physiology and Biophysics, Weill Medical College of Cornell Univ., 1300 York Ave., New York, NY 10021, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK-59659

ACRONYM: DK

MEDLINETA: Am J Physiol Renal Physiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Na channel expression and activity in the medullary collecting duct of rat kidney Related Publications

• A Markov model for relaxation and exchange in NMR spectroscopy.
• A missense mutation (c.184C>T) in ovine CLN6 causes neuronal ceroid lipofuscinosis in Merino sheep whereas affected South Hampshire sheep have reduced levels of CLN6 mRNA.
• Autophagy in the invading pathogen.
• Bioconversion of Chromene Derivatives in Seedlings of Ageratina adenophora.
• Chewing and food consistency: effects on bolus transport and swallow initiation.
• Conjugation of methoxypolyethylene glycol to the surface of bovine red blood cells.
• DNA vaccine construct incorporating intercellular trafficking and intracellular targeting motifs effectively primes and induces memory B- and T-cell responses in outbred animals.
• Diagnostic significance of Neospora caninum DNA detected by PCR in cattle serum.
• Dietary calcium and magnesium, major food sources, and risk of type 2 diabetes in U.S. black women.
• Distal clavicular osteolysis: MR evidence for subchondral fracture.
• Genetic diversity of Plasmodium vivax Pvcsp and Pvmsp1 in Guyana, South America.
• Hair relaxers not associated with breast cancer risk: evidence from the black women's health study.
• High O2 affinity hemoglobin-based oxygen carriers synthesized via polymerization of hemoglobin with ring-opened 2-chloroethyl-beta-D-fructopyranoside and 1-o-octyl-beta-D-glucopyranoside.
• Horizontal gene transfer in plants.
• How regulators of G protein signaling achieve selective regulation.
• Informed consent for schizophrenia research: what is an investigator (or IRB) to do?
• International comparison of C-peptide measurements.
• Joint composite-rotation adiabatic-sweep isotope filtration.
• Left-right patterning from the inside out: widespread evidence for intracellular control.
• Localization of the pH gate in Kir1.1 channels.
• Multiple method verification of the positional accuracy of linac based stereotactic radiosurgery and radiotherapy.
• Na channel expression and activity in the medullary collecting duct of rat kidney.
• Off-resonance TROSY-selected R 1rho experiment with improved sensitivity for medium- and high-molecular-weight proteins.
• Self-Assembled Poly(butadiene)-b-poly(ethylene oxide) Polymersomes as Paclitaxel Carriers.
• Socio-demographic and cultural comparison of overweight and obesity risk and prevalence in adolescents in Southern California and Wuhan, China.
• The effects of Ureplasma diversum inoculated into the amniotic cavity in cows.
• The twin-arginine translocation pathway is a major route of protein export in Streptomyces coelicolor.
• Time of ovarian follicular recruitment in cyclic pony mares.
• Use of chromosome substitution strains to identify seizure susceptibility loci in mice.
• Verbal fluency impairments among middle-aged and older outpatients with schizophrenia are characterized by deficient switching.