N-hexacosanol prevents diabetes-induced rat ileal dysfunction without qualitative alteration of the muscarinic receptor system.

N-hexacosanol prevents diabetes-induced rat ileal dysfunction without qualitative alteration of the muscarinic receptor system. Research Abstract Details 

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N-hexacosanol prevents diabetes-induced rat ileal dysfunction without qualitative alteration of the muscarinic receptor system. Abstract Text:

Naho Narimatsu,Motoaki Saito,Emi Kazuyama,Yoshie Hisadome,Yukako Kinoshita,Itaru Satoh,Shin-Ichi Okada,Hiroto Suzuki,Masashi Yamada,Keisuke Satoh,Naho Narimatsu,Motoaki Saito,Emi Kazuyama,Yoshie Hisadome,Yukako Kinoshita,Itaru Satoh,Shin-Ichi Okada,Hiroto Suzuki,Masashi Yamada,Keisuke Satoh,Naho Narimatsu,Motoaki Saito,Emi Kazuyama,Yoshie Hisadome,Yukako Kinoshita,Itaru Satoh,Shin-Ichi Okada,Hiroto Suzuki,Masashi Yamada,Keisuke Satoh,

We evaluated the effects of N-hexacosanol, a cyclohexenonic long-chain fatty alcohol, on muscarinic receptors in diabetic rat ileal dysfunction. Eight-week-old male SD rats were divided into four groups. After induction of diabetes (streptozotocin 50 mg/kg, i.p.), three groups were maintained for eight weeks with treatment by N-hexacosanol (0, 2 or 8 mg/kg, s.c. every day). Ileum function was investigated by organ bath studies using carbachol and KCl, and the expression levels of muscarinic M(2) and M(3) receptors were investigated by real-time polymerase chain reaction. Various concentrations of subtype-selective muscarinic antagonists, i.e., atropine (non-selective), pirenzepine (M(1) selective), methoctramine (M(2) selective), and 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP, M(1)/M(3) selective), were used in this study. In the presence and absence of these antagonists, contractile response curves to increasing concentrations of carbachol were investigated. Treatment with N-hexacosanol did not alter the diabetic status of the rats, but did significantly prevent the carbachol-induced hypercontractility in diabetic rat ileum. Estimation of the pA(2) values for atropine, pirenzepine, methoctramine, and 4-DAMP indicated that the carbacholinduced contractile response in the ileum is mainly mediated through the muscarinic M(3) receptor subtype in all groups. Furthermore, N-hexacosanol significantly prevented the diabetes-induced up-regulation of intestinal muscarinic M(2) and M(3) receptor mRNAs in streptozotocin-diabetic rats. Our data indicated that N-hexacosanol exerts preventive effects with respect to carbachol-induced hypercontractility in the diabetic rat ileum without qualitative alteration of the muscarinic receptor system.

N-hexacosanol prevents diabetes-induced rat ileal dysfunction without qualitative alteration of the muscarinic receptor system. Publishing Authors By Initials

N Narimatsu,M Saito,E Kazuyama,Y Hisadome,Y Kinoshita,I Satoh,S Okada,H Suzuki,M Yamada,K Satoh,N Narimatsu,M Saito,E Kazuyama,Y Hisadome,Y Kinoshita,I Satoh,S Okada,H Suzuki,M Yamada,K Satoh,N Narimatsu,M Saito,E Kazuyama,Y Hisadome,Y Kinoshita,I Satoh,S Okada,H Suzuki,M Yamada,K Satoh,

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N-hexacosanol prevents diabetes-induced rat ileal dysfunction without qualitative alteration of the muscarinic receptor system. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biomedical research (Tokyo, Japan)

VOLUME: 28

Page Numbers: 267-73

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ISSN: 0388-6107

DAY: 16

MONTH: Oct

YEAR: 2007

N-hexacosanol prevents diabetes-induced rat ileal dysfunction without qualitative alteration of the muscarinic receptor system. Information

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LANGUAGE: eng

NlmUniqueID: 8100317

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Grant and Affiliation Information for N-hexacosanol prevents diabetes-induced rat ileal dysfunction without qualitative alteration of the muscarinic receptor system.

AFFILIATION: Department of Pathophysiological and Therapeutic Science, Division of Molecular Pharmacology, Tottori University Faculty of Medicine, Nishimachi, Yonago.

Country: Japan

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MEDLINETA: Biomed Res

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