Myristoyl-based transport of peptides into living cells.

Myristoyl-based transport of peptides into living cells. Research Abstract Details 

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Myristoyl-based transport of peptides into living cells. Abstract Text:

Allison R Nelson,Laura Borland,Nancy L Allbritton,Christopher E Sims,Allison R Nelson,Laura Borland,Nancy L Allbritton,Christopher E Sims,

Translocation of membrane-impermeant molecules to the interior of living cells is a necessity for many biochemical investigations. Myristoylation was studied as a means to introduce peptides into living cells. Uptake of a myristoylated, fluorescent peptide was efficient in the B lymphocyte cell line BA/F3. In contrast, this cell line was resistant to uptake of a cell-penetrating peptide derived from the TAT protein. In BA/F3 cells, membrane association was shown to be rapid, reaching a maximum within 30 min. Cellular uptake of the peptide lagged the membrane association but occurred within a similar time frame. Experiments performed at 37 versus 4 degrees C demonstrated profound temperature dependence in the cellular uptake of myristoylated cargo. Myristoylated peptides with either positive or negative charge were shown to load efficiently. In contrast to TAT-conjugated cargo, pyrenebutyrate did not enhance cellular uptake of the myristoylated peptide. The myristoylated peptide did not adversely affect cell viability at concentrations up to 100 muM. This assessment of myristoyl-based transport provides fundamental data needed in understanding the intracellular delivery of myristoylated peptide cargoes for cell-based biochemical studies.

Myristoyl-based transport of peptides into living cells. Publishing Authors By Initials

AR Nelson,L Borland,NL Allbritton,CE Sims,AR Nelson,L Borland,NL Allbritton,CE Sims,

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Myristoyl-based transport of peptides into living cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biochemistry

VOLUME: 46

Page Numbers: 14771-81

Journal Abbreviation: Biochemistry

ISSN: 0006-2960

DAY: 29

MONTH: 11

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 370623

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Grant and Affiliation Information for Myristoyl-based transport of peptides into living cells.

AFFILIATION: Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599-3290, and Department of Physiology and Biophysics, University of California, Irvine, California 92697.

Country: United States

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MEDLINETA: Biochemistry

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