Myosin may stay in EADP species during the catch contraction in scallop smooth muscle.

Myosin may stay in EADP species during the catch contraction in scallop smooth muscle. Research Abstract Details 

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Myosin may stay in EADP species during the catch contraction in scallop smooth muscle. Abstract Text:

M Takahashi,F Morita,

Myosin (opaque myosin) isolated from the opaque portion of scallop smooth muscle, a catch muscle, was subjected to limited digestion by trypsin during the steady-state ATPase reaction. The 200-kDa heavy chain of opaque myosin was cleaved into 125- and 74-kDa fragments. The proteolytic rate in the absence of Ca2+ was lower than that in the presence of Ca2+, and was similar to that in the presence of ADP and absence of Ca2+. The results suggest that the steady-state intermediate of opaque myosin ATPase in the absence of Ca2+ is EADP, which is consistent with the previous results based on the difference UV-absorption spectrum (Takahashi, M., Sohma, H., & Morita, F. (1988) J. Biochem. 104, 102-107). In the presence of F-actin, the proteolytic rates were decreased, but the digestive patterns by trypsin were similar to those of myosin alone. Even in the presence of F-actin, the proteolytic rate during the ATPase reaction in the absence of Ca2+ was lower than that in the presence of Ca2+, and was similar to that in the presence of ADP and absence of Ca2+. In addition, there was another trypsin-susceptible site which is probably located at 18 kDa from the N-terminal of the heavy chain. The site in the absence of Ca2+ was hardly cleaved when ATP or ADP was present. Similar tendencies were observed even in the presence of F-actin. These findings suggest that the intermediate of opaque myosin ATPase at the steady state in the absence of Ca2+ is EADP even in the presence of F-actin.(ABSTRACT TRUNCATED AT 250 WORDS)

Myosin may stay in EADP species during the catch contraction in scallop smooth muscle. Publishing Authors By Initials

M Takahashi,F Morita,

For similar enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: trypsin research abstracts see: enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: trypsin research

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Myosin may stay in EADP species during the catch contraction in scallop smooth muscle. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 106

Page Numbers: 868-71

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1989

Myosin may stay in EADP species during the catch contraction in scallop smooth muscle. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Myosin may stay in EADP species during the catch contraction in scallop smooth muscle. Keywords Mesh Terms:

KEYWORDS: Trypsin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Myosin may stay in EADP species during the catch contraction in scallop smooth muscle. Information

Substance Name: Myosins

Registry Number: EC 3.6.1.4

Grant and Affiliation Information for Myosin may stay in EADP species during the catch contraction in scallop smooth muscle.

AFFILIATION: Department of Chemistry, Faculty of Science, Hokkaido University.

Country: JAPAN

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MEDLINETA: J Biochem

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