Myosin-like protein and actin-like protein from Escherichia coli K12 C600.

Myosin-like protein and actin-like protein from Escherichia coli K12 C600. Research Abstract Details 

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Myosin-like protein and actin-like protein from Escherichia coli K12 C600. Abstract Text:

K Nakamura,S Watanabe,

Myosin-like protein was obtained from E. coli by extraction with a sucrose solution and by precipitation with rabbit skeletal actin. The preparation of E. coli myosin-like protein looked very similar, in the sodium dodecyl sulfate-gel electrophoretic pattern, to that of rabbit skeletal myosin. The myosin-like protein was able to reversibly bind to rabbit actin. It had the activities of EDTA-, Ca-, and Mg-ATPases. The product in the EDTA-ATPase reaction catalyzed by the myosin-like protein was identified as ADP by ion exchange chromatography. The Mg-ATPase activity of E. coli myosin-like protein was activated by either rabbit actin or E. coli actin-like protein though the activation was much stronger by the latter. However, the myosin-like protein did not exhibit superprecipitation either with rabbit actin or with E. coli actin-like protein. Actin-like protein was also obtained from E. coli by essentially the same procedures as those described for preparation of rabbit skeletal actin. E. coli actin-like protein was capable of activating Mg-ATPase of rabbit myosin, and also of superprecipitation with rabbit myosin. Extraction from both the whole cells and the membrane fraction of E. coli strongly suggested that the myosin-like protein and the actin-like protein are both localized in the membrane fraction rather than in the cytoplasmic fraction.

Myosin-like protein and actin-like protein from Escherichia coli K12 C600. Publishing Authors By Initials

K Nakamura,S Watanabe,

For similar biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research abstracts see: biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research

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Myosin-like protein and actin-like protein from Escherichia coli K12 C600. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 83

Page Numbers: 1459-70

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1978

Myosin-like protein and actin-like protein from Escherichia coli K12 C600. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Myosin-like protein and actin-like protein from Escherichia coli K12 C600. Keywords Mesh Terms:

KEYWORDS: Species Specificity

MESH TERMS: metabolism

Chemical & Substance for Abstract: Myosin-like protein and actin-like protein from Escherichia coli K12 C600. Information

Substance Name: Myosins

Registry Number: EC 3.6.1.4

Grant and Affiliation Information for Myosin-like protein and actin-like protein from Escherichia coli K12 C600.

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Country: JAPAN

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MEDLINETA: J Biochem

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