Myosin cross-bridge kinetics and the mechanism of catch.

Myosin cross-bridge kinetics and the mechanism of catch. Research Abstract Details 

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Myosin cross-bridge kinetics and the mechanism of catch. Abstract Text:

Aaron S Franke,Susan U Mooers,Srinivasa R Narayan,Marion J Siegman,Thomas M Butler,

Catch force in molluscan smooth muscle requires little, if any, energy input and is controlled by the phosphorylation state of the thick filament-associated mini-titin, twitchin. The kinetic parameters of myosin cross-bridge turnover in permeabilized catch muscle, and how they are potentially modified by the catch mechanism, were determined by single turnover measurements on myosin-bound ADP. Under isometric conditions, there are fast and slow components of cross-bridge turnover that probably result from kinetic separation of calcium-bound and calcium-free cross-bridge pools. The structure responsible for catch force maintenance at intermediate [Ca+2] does not alter the processes responsible for the fast and slow components under isometric conditions. Also, there is no measurable turnover of myosin-bound ADP during relaxation of catch force by phosphorylation of twitchin at pCa > 8. The only effects of the catch link on myosin-bound ADP turnover are 1), a small, very slow extra turnover when catch force is maintained at very low [Ca+2] (pCa > 8); and 2), attenuation of the shortening-induced increase in turnover at subsaturating [Ca(+2)]. These limited interactions between the catch link and myosin cross-bridge turnover are consistent with the idea that catch force is maintained by a thick and thin filament linkage other than the myosin cross-bridge.

Myosin cross-bridge kinetics and the mechanism of catch. Publishing Authors By Initials

AS Franke,SU Mooers,SR Narayan,MJ Siegman,TM Butler,

For similar biochemical phenomena, metabolism, and nutrition: metabolism: phosphorylation research abstracts see: biochemical phenomena, metabolism, and nutrition: metabolism: phosphorylation research

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Myosin cross-bridge kinetics and the mechanism of catch. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biophysical journal

VOLUME: 93

Page Numbers: 554-65

Journal Abbreviation: Biophys. J.

ISSN: 0006-3495

DAY: 27

MONTH: 04

YEAR: 2007

Myosin cross-bridge kinetics and the mechanism of catch. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370626

Myosin cross-bridge kinetics and the mechanism of catch. Keywords Mesh Terms:

KEYWORDS: Phosphorylation

MESH TERMS: physiology

Chemical & Substance for Abstract: Myosin cross-bridge kinetics and the mechanism of catch. Information

Substance Name: Myosins

Registry Number: EC 3.6.1.4

Grant and Affiliation Information for Myosin cross-bridge kinetics and the mechanism of catch.

AFFILIATION: Department of Molecular Physiology and Biophysics, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: T32HL07599

ACRONYM: HL

MEDLINETA: Biophys J

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