Myoglobin modification by enzyme-generated dopamine reactive species.

Myoglobin modification by enzyme-generated dopamine reactive species. Research Abstract Details 

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Myoglobin modification by enzyme-generated dopamine reactive species. Abstract Text:

The generation of reactive quinone species (DAQ) from oxidation of dopamine (DA) is involved in neurodegenerative pathologies like Parkinson's disease (A. Borta, G. U. Höglinger, J. Neurochem. 2007, 100, 587-595). The oxidation of DA to DAQ can occur either in a single two-electron process or in two consecutive one-electron steps, through semiquinone radicals, giving rise to different patterns of reactions. The former type of reaction can be promoted by tyrosinase, the latter by peroxidases in the presence of H(2)O(2), which can be formed under oxidative stress conditions. Both enzymes were employed for the characterization of the thiol-catechol adducts formed by reaction of DA and cysteine or glutathione, and for the identification of specific amino acid residues modified by DAQs in two representative target proteins, human and horse heart myoglobin. Our results indicate that the cysteinyl-DA adducts are formed from the same quinone intermediate independently of the mechanism of DA oxidation, and that the hallmark of a radical mechanism is the formation of the cystine dimer. The reactivity of quinone species also controls the DA-promoted derivatization of histidine residues in proteins. However, for the modification of the cysteine residue in human myoglobin, a radical intramolecular mechanism has been proposed, in which the protein acts both as the catalyst and target of the reaction. Most importantly, the modification of myoglobins through DAQ linkages, and in particular by DA oligomers, has dramatic effects on their stability, as it induces protein unfolding and incorporation into insoluble melanic precipitates.

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Myoglobin modification by enzyme-generated dopamine reactive species. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Chemistry (Weinheim an der Bergstrasse, Germany)

VOLUME: 14

Page Numbers: 8661-73

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ISSN: 0947-6539

DAY: 1

MONTH: 10

YEAR: 2008

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LANGUAGE: eng

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Grant and Affiliation Information for Myoglobin modification by enzyme-generated dopamine reactive species.

AFFILIATION: Dipartimento di Chimica Generale, Università di Pavia, Via Taramelli 12, 27100 Pavia (Italy), Fax: (+39) 0382-528544.

Country: Germany

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MEDLINETA: Chemistry

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