Myelopoiesis in the zebrafish, Danio rerio.

Myelopoiesis in the zebrafish, Danio rerio. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Myelopoiesis in the zebrafish, Danio rerio. Abstract Text:

Genome-wide chemical mutagenesis screens in the zebrafish (Danio rerio) have led to the identification of novel genes affecting vertebrate erythropoiesis. In determining if this approach could also be used to clarify the molecular genetics of myelopoiesis, it was found that the developmental hierarchy of myeloid precursors in the zebrafish kidney is similar to that in human bone marrow. Zebrafish neutrophils resembled human neutrophils, possessing segmented nuclei and myeloperoxidase-positive cytoplasmic granules. The zebrafish homologue of the human myeloperoxidase (MPO) gene, which is specific to cells of the neutrophil lineage, was cloned and used to synthesize antisense RNA probes for in situ hybridization analyses of zebrafish embryos. Granulocytic cells expressing zebrafish mpo were first evident at 18 hours after fertilization (hpf) in the posterior intermediate cell mass (ICM) and on the anterior yolk sac by 20 hpf. By 24 hpf, mpo-expressing cells were observed along the ICM and within the developing vascular system. Thus, the mpo gene should provide a useful molecular probe for identifying zebrafish mutants with defects in granulopoiesis. The expression of zebrafish homologues was also examined in 2 other mammalian hematopoietic genes, Pu.1, which appears to initiate a commitment step in normal mammalian myeloid development, and L-Plastin, a gene expressed by human monocytes and macrophages. The results demonstrate a high level of conservation of the spatio-temporal expression patterns of these genes between zebrafish and mammals. The morphologic and molecular genetic evidence presented here supports the zebrafish as an informative model system for the study of normal and aberrant human myelopoiesis. (Blood. 2001;98:643-651)

Myelopoiesis in the zebrafish, Danio rerio. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Myelopoiesis in the zebrafish, Danio rerio. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Blood

VOLUME: 98

Page Numbers: 643-51

Journal Abbreviation: Blood

ISSN: 0006-4971

DAY: 1

MONTH: Aug

YEAR: 2001

Myelopoiesis in the zebrafish, Danio rerio. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7603509

Myelopoiesis in the zebrafish, Danio rerio. Keywords Mesh Terms:

KEYWORDS: Zebrafish

MESH TERMS: physiology

Chemical & Substance for Abstract: Myelopoiesis in the zebrafish, Danio rerio. Information

Substance Name: Peroxidase

Registry Number: EC 1.11.1.7

Grant and Affiliation Information for Myelopoiesis in the zebrafish, Danio rerio.

AFFILIATION: Department of Pediatric Oncology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK 02593

ACRONYM: DK

MEDLINETA: Blood

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER: AF349034

Number Hits: 0

Myelopoiesis in the zebrafish, Danio rerio Related Publications

• A creative response meets community need/enriches hospital.
• Activating mutations in ALK provide a therapeutic target in neuroblastoma.
• Acute lymphoblastic leukaemia.
• Chk1 Suppresses a Caspase-2 Apoptotic Response to DNA Damage that Bypasses p53, Bcl-2, and Caspase-3.
• Chromosome 5q deletion and epigenetic suppression of the gene encoding alpha-catenin (CTNNA1) in myeloid cell transformation.
• Combined source-detector response characteristics.
• Dominant-interfering C/EBPalpha stimulates primitive erythropoiesis in zebrafish.
• FBW7 mutations in leukemic cells mediate NOTCH pathway activation and resistance to gamma-secretase inhibitors.
• Identification of structural aberrations in cancer by SNP array analysis.
• Inhibition of Jak1-dependent signal transduction in airway epithelial cells infected with adenovirus.
• Mechanisms of transcription factor deregulation in lymphoid cell transformation.
• Myelopoiesis in the zebrafish, Danio rerio.
• NOTCH and PI3K-AKT pathways intertwined.
• Type 2 diabetes in native Hawaiians and Pacific Islanders in Hawaii.