Myelin proteolipid protein (PLP) as a marker antigen of central nervous system contaminations for routine food control.

Myelin proteolipid protein (PLP) as a marker antigen of central nervous system contaminations for routine food control. Research Abstract Details 

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Myelin proteolipid protein (PLP) as a marker antigen of central nervous system contaminations for routine food control. Abstract Text:

Carmen Villmann,Barbara Sandmeier,Silke Seeber,Ewald Hannappel,Monika Pischetsrieder,Cord-Michael Becker,

Spreading transmissible spongiform encephalopathies (TSE) have been widely attributed to transmission by ingestion of mammalian central nervous system (CNS) tissue. Reliable exclusion of this epidemiological important route of transmission relies on an effective surveillance of food contamination. Here, myelin proteolipid protein (PLP) is identified as a specific and largely heat-resistant marker for detection of food contaminations by CNS tissue. PLP is a component of oligodendritic glial sheaths of neuronal processes that is specifically expressed in the CNS. A highly selective polyclonal antibody was developed directed against an epitope present in the full-length PLP protein, but absent from the developmentally regulated splice variant DM-20. In combination with a hydrophobic extraction of PLP from tissue samples, the antibody reliably detected PLP from spinal cord, cerebellum, and cortex of different mammalian species. Consistent with earlier reports on PLP expression, no cross-reactivity was observed with peripheral nerve or extraneural tissue, except for a very faint signal obtained with heart. When applied to an artificial CNS contamination present in sausages, the antibody reliably detected a low concentration (1%) of the contaminant. Application of heat, as used during conventional sausage manufacturing, led to a predominant alteration of arginine residues in the PLP protein and a partial loss of immunoreactivity. In contrast, a stretch of hydrophilic amino acids(112-122) proved to be heat-resistant, preserving the immunogenicity of this PLP epitope during heating. Taken together, the excellent CNS specificity of PLP immunodetection and the presence of a heat-resistant epitope have permitted the development of a highly sensitive immunoassay for CNS contamination in routine food control.

Myelin proteolipid protein (PLP) as a marker antigen of central nervous system contaminations for routine food control. Publishing Authors By Initials

C Villmann,B Sandmeier,S Seeber,E Hannappel,M Pischetsrieder,CM Becker,

For similar biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research abstracts see: biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research

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Myelin proteolipid protein (PLP) as a marker antigen of central nervous system contaminations for routine food control. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of agricultural and food chemistry

VOLUME: 55

Page Numbers: 7114-23

Journal Abbreviation: J. Agric. Food Chem.

ISSN: 0021-8561

DAY: 13

MONTH: 07

YEAR: 2007

Myelin proteolipid protein (PLP) as a marker antigen of central nervous system contaminations for routine food control. Information

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LANGUAGE: eng

NlmUniqueID: 374755

Myelin proteolipid protein (PLP) as a marker antigen of central nervous system contaminations for routine food control. Keywords Mesh Terms:

KEYWORDS: Species Specificity

MESH TERMS: immunology

Chemical & Substance for Abstract: Myelin proteolipid protein (PLP) as a marker antigen of central nervous system contaminations for routine food control. Information

Substance Name: Myelin Proteolipid Protein

Registry Number: 0

Grant and Affiliation Information for Myelin proteolipid protein (PLP) as a marker antigen of central nervous system contaminations for routine food control.

AFFILIATION: Institut für Biochemie, Emil-Fischer-Zentrum, Friedrich-Alexander-Universität Erlangen-Nürnberg, Fahrstrasse 17, 91054 Erlangen, Germany.

Country: United States

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MEDLINETA: J Agric Food Chem

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