Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: insights from genome-wide transcriptional profiling.

Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: insights from genome-wide transcriptional profiling. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: insights from genome-wide transcriptional profiling. Abstract Text:

Adel M Talaat,Sarah K Ward,Chia-Wei Wu,Elizabeth Rondon,Christine Tavano,John P Bannantine,Rick Lyons,Stephen A Johnston,

Chronic tuberculosis represents a major health problem for one-third of the world's population today. A key question relevant to chronic tuberculosis is the physiological status of Mycobacterium tuberculosis during this important stage of infection. To examine the molecular bases of chronic tuberculosis and the role of host immunity in mycobacterial growth, we determined the mycobacterial transcriptional profiles during chronic and reactivation phases of murine tuberculosis using in vivo microarray analysis (IVMA). Following 28 days of aerosol infection, mycobacterial counts remained stable, although the bacilli were metabolically active with a 50% active transcriptome. The expression of genes involved in lipid and carbohydrate pathways was significantly enriched during the middle stage of chronic tuberculosis, suggesting a nutrient-rich microenvironment. A total of 137 genes were significantly regulated in mid-chronic tuberculosis (45 and 60 days) compared to an early stage (14 days) of infection. Additional sets of genes, including the virulence regulator virS, were up-regulated during the reactivation stage, indicating their possible roles in mycobacterial resurgence. Interestingly, a set of potential transcriptional regulators was significantly induced at the late stage of chronic tuberculosis. Bioinformatic analysis identified a large number of genes that could be regulated by one of the potential transcriptional regulators encoded by rv0348, including the sigF operon. Taken together, IVMA provided a better definition of the transcriptional machinery activated during chronic and reactivation stages of tuberculosis and identified a novel transcriptional regulator. A similar approach can be adopted to study key stages of intracellular pathogens.

Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: insights from genome-wide transcriptional profiling. Publishing Authors By Initials

AM Talaat,SK Ward,CW Wu,E Rondon,C Tavano,JP Bannantine,R Lyons,SA Johnston,

For similar bacterial infections and mycoses: bacterial infections: gram-positive bacterial infections: actinomycetales infections: mycobacterium infections: tuberculosis: tuberculosis, pulmonary research abstracts see: bacterial infections and mycoses: bacterial infections: gram-positive bacterial infections: actinomycetales infections: mycobacterium infections: tuberculosis: tuberculosis, pulmonary research

PUBMED ID PMID:

MEDLINE DATE:

Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: insights from genome-wide transcriptional profiling. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 4265-74

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 23

MONTH: 03

YEAR: 2007

Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: insights from genome-wide transcriptional profiling. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: insights from genome-wide transcriptional profiling. Keywords Mesh Terms:

KEYWORDS: Tuberculosis, Pulmonary

MESH TERMS: pathology

Chemical & Substance for Abstract: Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: insights from genome-wide transcriptional profiling. Information

Substance Name: Bacterial Proteins

Registry Number: 0

Grant and Affiliation Information for Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: insights from genome-wide transcriptional profiling.

AFFILIATION: Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI 53706-1581, USA. atalaat@wisc.edu

Country: United States

AGENCY: United States NIGMS

GRANT: T32GM007215

ACRONYM: GM

MEDLINETA: J Bacteriol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: insights from genome-wide transcriptional profiling Related Publications

• Cancer cachexia - A clinical entity?
• Comparison of body protein metabolism during total parenteral nutrition using glucose or glucose and fat as the energy source.
• Cross-cultural differences in beliefs and practices that affect the language spoken to children: mothers with Indian and Western heritage.
• Deletion of Kv4.2 gene eliminates dendritic A-type K+ current and enhances induction of long-term potentiation in hippocampal CA1 pyramidal neurons.
• Dominant-subordinate relationships in hamsters: sex differences in reactions to familiar opponents.
• Foreign bodies in the air and food passages.
• Impact of comorbidities on the measurement of health in patients with ankle osteoarthritis.
• Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: insights from genome-wide transcriptional profiling.
• Osteopathic manipulative treatment of a 27-year-old man after anterior cruciate ligament reconstruction.
• Possible four-electrode configurations for measuring cardiac tissue fiber rotation.
• Post-operative fatigue - a real phenomenon attributable to the metabolic effects of surgery on body nutritional stores.
• Preliminary investigation of breast tumor detection using cross-vivaldi antenna.
• Protein kinase R, IkappaB kinase-beta and NF-kappaB are required for human rhinovirus induced pro-inflammatory cytokine production in bronchial epithelial cells.
• Relationship Between Vitamin C Status and Respiratory Exchange Ratio During Submaximal Exercise in Healthy Adults: 2488: Board #35 June 2 8:00 AM - 9:30 AM.
• Relationships between growth and retention of dietary lipids in juvenile southern rock lobster Jasus edwardsii.
• Role of deficient type III interferon-lambda production in asthma exacerbations.
• State-dependent modulation of amygdala inputs by dopamine-induced enhancement of sodium currents in layer V entorhinal cortex.
• Synthesis of an advanced intermediate en route to the mitomycin natural products.
• Systematic reviews from the cochrane musculoskeletal group.
• The infant with respiratory stridor.
• The need to include BIPP reactions in routine consent.
• The rat epididymal transcriptome: comparison of segmental gene expression in the rat and mouse epididymides.
• The role of barium swallow in the management of the globus pharyngeus.
• The role of spermidine/spermine N1-acetyltransferase in determining response to chemotherapeutic agents in colorectal cancer cells.
• The role of the proteasomal ATPases and activator monoubiquitylation in regulating Gal4 binding to promoters.
• The thin child.
• Total hip arthroplasty with a cemented, polished, collared femoral stem and a cementless acetabular component. A follow-up study at a minimum of ten years.
• Vaccination with Venezuelan equine encephalitis replicons encoding cowpox virus structural proteins protects mice from intranasal cowpox virus challenge.
• Vitamin C Status is Directly Associated with Brachial Artery Distensibility Following Maximal Exercise in Adults: 1689: Board #179 May 30 2:00 PM - 3:30 PM.
• shRNAs targeting hepatitis C: effects of sequence and structural features, and comparision with siRNA.