MYB98 positively regulates a battery of synergid-expressed genes encoding filiform apparatus localized proteins.

MYB98 positively regulates a battery of synergid-expressed genes encoding filiform apparatus localized proteins. Research Abstract Details 

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MYB98 positively regulates a battery of synergid-expressed genes encoding filiform apparatus localized proteins. Abstract Text:

Jayson A Punwani,David S Rabiger,Gary N Drews,Jayson A Punwani,David S Rabiger,Gary N Drews,

The synergid cells within the female gametophyte are essential for reproduction in angiosperms. MYB98 encodes an R2R3-MYB protein required for pollen tube guidance and filiform apparatus formation by the synergid cells. To test the predicted function of MYB98 as a transcriptional regulator, we determined its subcellular localization and examined its DNA binding properties. We show that MYB98 binds to a specific DNA sequence (TAAC) and that a MYB98-green fluorescent protein fusion protein localizes to the nucleus, consistent with a role in transcriptional regulation. To identify genes regulated by MYB98, we tested previously identified synergid-expressed genes for reduced expression in myb98 female gametophytes and identified 16 such genes. We dissected the promoter of one of the downstream genes, DD11, and show that it contains a MYB98 binding site required for synergid expression, suggesting that DD11 is regulated directly by MYB98. To gain insight into the functions of the downstream genes, we chose five genes and determined the subcellular localization of the encoded proteins. We show that these five proteins are secreted into the filiform apparatus, suggesting that they play a role in either the formation or the function of this unique structure. Together, these data suggest that MYB98 functions as a transcriptional regulator in the synergid cells and activates the expression of genes required for pollen tube guidance and filiform apparatus formation.

MYB98 positively regulates a battery of synergid-expressed genes encoding filiform apparatus localized proteins. Publishing Authors By Initials

JA Punwani,DS Rabiger,GN Drews,JA Punwani,DS Rabiger,GN Drews,

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MYB98 positively regulates a battery of synergid-expressed genes encoding filiform apparatus localized proteins. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Plant cell

VOLUME: 19

Page Numbers: 2557-68

Journal Abbreviation: Plant Cell

ISSN: 1040-4651

DAY: 10

MONTH: 08

YEAR: 2007

MYB98 positively regulates a battery of synergid-expressed genes encoding filiform apparatus localized proteins. Information

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LANGUAGE: eng

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Grant and Affiliation Information for MYB98 positively regulates a battery of synergid-expressed genes encoding filiform apparatus localized proteins.

AFFILIATION: Department of Biology, University of Utah, Salt Lake City, Utah 84112-0840, USA.

Country: United States

AGENCY: United States NICHD

GRANT: 5T32 HD-07491

ACRONYM: HD

MEDLINETA: Plant Cell

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