Mutations in West Nile virus nonstructural proteins that facilitate replicon persistence in vitro attenuate virus replication in vitro and in vivo.

Mutations in West Nile virus nonstructural proteins that facilitate replicon persistence in vitro attenuate virus replication in vitro and in vivo. Research Abstract Details 

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Mutations in West Nile virus nonstructural proteins that facilitate replicon persistence in vitro attenuate virus replication in vitro and in vivo. Abstract Text:

Shannan L Rossi,Rafik Fayzulin,Nathan Dewsbury,Nigel Bourne,Peter W Mason,

West Nile virus (WNV) infections in vertebrates are generally acute but persistent infections have been observed. To investigate the ability of WNV to produce persistent infections, we forced subgenomic WNV replicons to replicate within a cell without causing cell death. Detailed analyses of these cell-adapted genomes revealed mutations within the nonstructural protein genes NS2A (D73H, M108K), NS3 (117Kins), NS4B (E249G) and NS5 (P528H). WNV replicons and WNVs harboring a subset of NS2A or NS3 mutations showed a reduction in genome replication, a reduction in antigen accumulation, a decrease in cytopathic effect, an increased ability to persist in cell culture and/or attenuation in vivo. Taken together, these data indicate that WNV with a defect in replication and an increased potential to persist within the host cell can be generated by point mutations at multiple independent loci, suggesting that persistent viruses could arise in nature.

Mutations in West Nile virus nonstructural proteins that facilitate replicon persistence in vitro attenuate virus replication in vitro and in vivo. Publishing Authors By Initials

SL Rossi,R Fayzulin,N Dewsbury,N Bourne,PW Mason,

For similar viruses: rna viruses: flaviviridae: flavivirus: encephalitis viruses, japanese: west nile virus research abstracts see: viruses: rna viruses: flaviviridae: flavivirus: encephalitis viruses, japanese: west nile virus research

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Mutations in West Nile virus nonstructural proteins that facilitate replicon persistence in vitro attenuate virus replication in vitro and in vivo. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Virology

VOLUME: 364

Page Numbers: 184-95

Journal Abbreviation: Virology

ISSN: 0042-6822

DAY: 23

MONTH: 03

YEAR: 2007

Mutations in West Nile virus nonstructural proteins that facilitate replicon persistence in vitro attenuate virus replication in vitro and in vivo. Information

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LANGUAGE: eng

NlmUniqueID: 110674

Mutations in West Nile virus nonstructural proteins that facilitate replicon persistence in vitro attenuate virus replication in vitro and in vivo. Keywords Mesh Terms:

KEYWORDS: West Nile virus

MESH TERMS: physiology

Chemical & Substance for Abstract: Mutations in West Nile virus nonstructural proteins that facilitate replicon persistence in vitro attenuate virus replication in vitro and in vivo. Information

Substance Name: Interferons

Registry Number: 9008-11-1

Grant and Affiliation Information for Mutations in West Nile virus nonstructural proteins that facilitate replicon persistence in vitro attenuate virus replication in vitro and in vivo.

AFFILIATION: Department of Pathology, 3.206B Mary Moody Northen Pavilion, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-0436, USA.

Country: United States

AGENCY: United States NIAID

GRANT: U54 AI057156

ACRONYM: AI

MEDLINETA: Virology

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