Mutation in mouse hei10, an e3 ubiquitin ligase, disrupts meiotic crossing over.

Mutation in mouse hei10, an e3 ubiquitin ligase, disrupts meiotic crossing over. Research Abstract Details 

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Mutation in mouse hei10, an e3 ubiquitin ligase, disrupts meiotic crossing over. Abstract Text:

Jeremy O Ward,Laura G Reinholdt,William W Motley,Lisa M Niswander,Dekker C Deacon,Laurie B Griffin,Kristofor K Langlais,Vickie L Backus,Kerry J Schimenti,Marilyn J O'Brien,John J Eppig,John C Schimenti,Jeremy O Ward,Laura G Reinholdt,William W Motley,Lisa M Niswander,Dekker C Deacon,Laurie B Griffin,Kristofor K Langlais,Vickie L Backus,Kerry J Schimenti,Marilyn J O'Brien,John J Eppig,John C Schimenti,

Crossing over during meiotic prophase I is required for sexual reproduction in mice and contributes to genome-wide genetic diversity. Here we report on the characterization of an N-ethyl-N-nitrosourea-induced, recessive allele called mei4, which causes sterility in both sexes owing to meiotic defects. In mutant spermatocytes, chromosomes fail to congress properly at the metaphase plate, leading to arrest and apoptosis before the first meiotic division. Mutant oocytes have a similar chromosomal phenotype but in vitro can undergo meiotic divisions and fertilization before arresting. During late meiotic prophase in mei4 mutant males, absence of cyclin dependent kinase 2 and mismatch repair protein association from chromosome cores is correlated with the premature separation of bivalents at diplonema owing to lack of chiasmata. We have identified the causative mutation, a transversion in the 5' splice donor site of exon 1 in the mouse ortholog of Human Enhancer of Invasion 10 (Hei10; also known as Gm288 in mouse and CCNB1IP1 in human), a putative B-type cyclin E3 ubiquitin ligase. Importantly, orthologs of Hei10 are found exclusively in deuterostomes and not in more ancestral protostomes such as yeast, worms, or flies. The cloning and characterization of the mei4 allele of Hei10 demonstrates a novel link between cell cycle regulation and mismatch repair during prophase I.

Mutation in mouse hei10, an e3 ubiquitin ligase, disrupts meiotic crossing over. Publishing Authors By Initials

JO Ward,LG Reinholdt,WW Motley,LM Niswander,DC Deacon,LB Griffin,KK Langlais,VL Backus,KJ Schimenti,MJ O'Brien,JJ Eppig,JC Schimenti,JO Ward,LG Reinholdt,WW Motley,LM Niswander,DC Deacon,LB Griffin,KK Langlais,VL Backus,KJ Schimenti,MJ O'Brien,JJ Eppig,JC Schimenti,

For similar enzymes and coenzymes: enzymes: ligases: ubiquitin-protein ligase complexes: ubiquitin-protein ligases research abstracts see: enzymes and coenzymes: enzymes: ligases: ubiquitin-protein ligase complexes: ubiquitin-protein ligases research

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Mutation in mouse hei10, an e3 ubiquitin ligase, disrupts meiotic crossing over. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: PLoS genetics

VOLUME: 3

Page Numbers: e139

Journal Abbreviation: PLoS Genet.

ISSN: 1553-7404

DAY: 6

MONTH: 07

YEAR: 2007

Mutation in mouse hei10, an e3 ubiquitin ligase, disrupts meiotic crossing over. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101239074

Mutation in mouse hei10, an e3 ubiquitin ligase, disrupts meiotic crossing over. Keywords Mesh Terms:

KEYWORDS: Ubiquitin-Protein Ligases

MESH TERMS: physiology

Chemical & Substance for Abstract: Mutation in mouse hei10, an e3 ubiquitin ligase, disrupts meiotic crossing over. Information

Substance Name: Ubiquitin-Protein Ligases

Registry Number: EC 6.3.2.19

Grant and Affiliation Information for Mutation in mouse hei10, an e3 ubiquitin ligase, disrupts meiotic crossing over.

AFFILIATION: Middlebury College, Middlebury, Vermont, United States of America. jward@middlebury.edu

Country: United States

AGENCY: United States NIGMS

GRANT: R15 GM078183–01

ACRONYM: GM

MEDLINETA: PLoS Genet

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