Mutagenesis of the C1 Oxidation Pathway in Methanosarcina barkeri: New Insights into the Mtr/Mer Bypass Pathway.

Mutagenesis of the C1 Oxidation Pathway in Methanosarcina barkeri: New Insights into the Mtr/Mer Bypass Pathway. Research Abstract Details 

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Mutagenesis of the C1 Oxidation Pathway in Methanosarcina barkeri: New Insights into the Mtr/Mer Bypass Pathway. Abstract Text:

A series of Methanosarcina barkeri mutants lacking the genes encoding the enzymes involved in the C1 oxidation/reduction pathway were constructed. Mutants lacking the methyl-tetrahydromethanopterin (H(4)MPT):coenzyme M (CoM) methyltransferase-encoding operon (Deltamtr), the methylene-H(4)MPT reductase-encoding gene (Deltamer), the methylene-H(4)MPT dehydrogenase-encoding gene (Deltamtd), and the formyl-methanofuran:H(4)MPT formyl-transferase-encoding gene (Deltaftr) all failed to grow using either methanol or H(2)/CO(2) as a growth substrate, indicating that there is an absolute requirement for the C1 oxidation/reduction pathway for hydrogenotrophic and methylotrophic methanogenesis. The mutants also failed to grow on acetate, and we suggest that this was due to an inability to generate the reducing equivalents needed for biosynthetic reactions. Despite their lack of growth on methanol, the Deltamtr and Deltamer mutants were capable of producing methane from this substrate, whereas the Deltamtd and Deltaftr mutants were not. Thus, there is an Mtr/Mer bypass pathway that allows oxidation of methanol to the level of methylene-H(4)MPT in M. barkeri. The data further suggested that formaldehyde may be an intermediate in this bypass; however, no methanol dehydrogenase activity was found in Deltamtr cell extracts, nor was there an obligate role for the formaldehyde-activating enzyme (Fae), which has been shown to catalyze the condensation of formaldehyde and H(4)MPT in vitro. Both the Deltamer and Deltamtr mutants were able to grow on a combination of methanol plus acetate, but they did so by metabolic pathways that are clearly distinct from each other and from previously characterized methanogenic pathways.

Mutagenesis of the C1 Oxidation Pathway in Methanosarcina barkeri: New Insights into the Mtr/Mer Bypass Pathway. Publishing Authors By Initials

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Mutagenesis of the C1 Oxidation Pathway in Methanosarcina barkeri: New Insights into the Mtr/Mer Bypass Pathway. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of bacteriology

VOLUME: 190

Page Numbers: 1928-36

Journal Abbreviation: J. Bacteriol.

ISSN: 1098-5530

DAY: 4

MONTH: 01

YEAR: 2008

Mutagenesis of the C1 Oxidation Pathway in Methanosarcina barkeri: New Insights into the Mtr/Mer Bypass Pathway. Information

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LANGUAGE: eng

NlmUniqueID: 2985120

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AFFILIATION: Department of Microbiology, University of Illinois at Urbana-Champaign, 601 S. Goodwin Avenue, Urbana, IL 61801. metcalf@uiuc.edu.

Country: United States

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MEDLINETA: J Bacteriol

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