Multisubunit RNA polymerases melt only a single DNA base pair downstream of the active site.

Multisubunit RNA polymerases melt only a single DNA base pair downstream of the active site. Research Abstract Details 

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Multisubunit RNA polymerases melt only a single DNA base pair downstream of the active site. Abstract Text:

Ekaterina Kashkina,Michael Anikin,Florian Brueckner,Elisabeth Lehmann,Sergey N Kochetkov,William T McAllister,Patrick Cramer,Dmitry Temiakov,

To extend the nascent transcript, RNA polymerases must melt the DNA duplex downstream from the active site to expose the next acceptor base for substrate binding and incorporation. A number of mechanisms have been proposed to account for the manner in which the correct substrate is selected, and these differ in their predictions as to how far the downstream DNA is melted. Using fluorescence quenching experiments, we provide evidence that cellular RNA polymerases from bacteria and yeast melt only one DNA base pair downstream from the active site. These data argue against a model in which multiple NTPs are lined up downstream of the active site.

Multisubunit RNA polymerases melt only a single DNA base pair downstream of the active site. Publishing Authors By Initials

E Kashkina,M Anikin,F Brueckner,E Lehmann,SN Kochetkov,WT McAllister,P Cramer,D Temiakov,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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Multisubunit RNA polymerases melt only a single DNA base pair downstream of the active site. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 21578-82

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 25

MONTH: 05

YEAR: 2007

Multisubunit RNA polymerases melt only a single DNA base pair downstream of the active site. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

Multisubunit RNA polymerases melt only a single DNA base pair downstream of the active site. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Multisubunit RNA polymerases melt only a single DNA base pair downstream of the active site. Information

Substance Name: DNA-Directed RNA Polymerases

Registry Number: EC 2.7.7.6

Grant and Affiliation Information for Multisubunit RNA polymerases melt only a single DNA base pair downstream of the active site.

AFFILIATION: Department of Cell Biology, University of Medicine and Dentistry of New Jersey, School of Osteopathic Medicine, Stratford, NJ 08084, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM38147

ACRONYM: GM

MEDLINETA: J Biol Chem

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