Multiplexed high-throughput electrokinetically-controlled immunoassay for the detection of specific bacterial antibodies in human serum.

Multiplexed high-throughput electrokinetically-controlled immunoassay for the detection of specific bacterial antibodies in human serum. Research Abstract Details 

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Multiplexed high-throughput electrokinetically-controlled immunoassay for the detection of specific bacterial antibodies in human serum. Abstract Text:

Yali Gao,Philip M Sherman,Yu Sun,Dongqing Li,Yali Gao,Philip M Sherman,Yu Sun,Dongqing Li,Yali Gao,Philip M Sherman,Yu Sun,Dongqing Li,

In previous studies we have developed a simple electrokinetically-controlled lab-on-a-chip for heterogeneous immunoassay. In that method, all the sequential operations in an immunoassay, such as reagent loading and washing, were performed automatically by electrokinetically controlling the flow in an H-shaped microchannel. Here, we demonstrated further development of a high-throughput immunoassay microfluidic chip, and the application of the new immunoassay microfluidic chip in assaying human serum. The microfluidic immunoassay analyzed ten samples in parallel in 22min. Bacterial antibodies in samples were captured by antigens pre-patterned on the bottom wall of a microchannel and then bound with TRITC-labeled detection antibodies to generate fluorescent signals. With optimized surface concentration of antigen, the assay detected Escherichia coli O157:H7 antibody and Helicobacter pylori antibody from buffer solutions in concentration ranges of 0.02-10mugmL(-1) and 0.1-50mugmL(-1), respectively. Human sera that were E. coli-positive or H. pylori-positive were accurately distinguished from respective negative controls. Moreover, the two antibodies, anti-E. coli and anti- H. pylori antibodies, could be simultaneously detected from human serum. This electrokinetically-controlled immunoassay shows an excellent potential for efficiently detecting multiple pathogenic infections in clinical environments.

Multiplexed high-throughput electrokinetically-controlled immunoassay for the detection of specific bacterial antibodies in human serum. Publishing Authors By Initials

Y Gao,PM Sherman,Y Sun,D Li,Y Gao,PM Sherman,Y Sun,D Li,Y Gao,PM Sherman,Y Sun,D Li,

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Multiplexed high-throughput electrokinetically-controlled immunoassay for the detection of specific bacterial antibodies in human serum. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytica chimica acta

VOLUME: 606

Page Numbers: 98-107

Journal Abbreviation: Anal. Chim. Acta

ISSN: 1873-4324

DAY: 6

MONTH: 11

YEAR: 2007

Multiplexed high-throughput electrokinetically-controlled immunoassay for the detection of specific bacterial antibodies in human serum. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Multiplexed high-throughput electrokinetically-controlled immunoassay for the detection of specific bacterial antibodies in human serum.

AFFILIATION: Department of Mechanical and Industrial Engineering, University of Toronto, 5 King's College Road, Toronto, Ontario M5S 3G8, Canada.

Country: Netherlands

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MEDLINETA: Anal Chim Acta

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