Multiple upstream modules regulate zebrafish myf5 expression.

Multiple upstream modules regulate zebrafish myf5 expression. Research Abstract Details 

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Multiple upstream modules regulate zebrafish myf5 expression. Abstract Text:

Yau-Hung Chen,Yun-Hsin Wang,Min-Yen Chang,Cheng-Yung Lin,Chih-Wei Weng,Monte Westerfield,Huai-Jen Tsai,

BACKGROUND: Myf5 is one member of the basic helix-loop-helix family of transcription factors, and it functions as a myogenic factor that is important for the specification and differentiation of muscle cells. The expression of myf5 is somite- and stage-dependent during embryogenesis through a delicate regulation. However, this complex regulatory mechanism of myf5 is not clearly understood. RESULTS: We isolated a 156-kb bacterial artificial chromosome clone that includes an upstream 80-kb region and a downstream 70-kb region of zebrafish myf5 and generated a transgenic line carrying this 156-kb segment fused to a green fluorescent protein (GFP) reporter gene. We find strong GFP expression in the most rostral somite and in the presomitic mesoderm during segmentation stages, similar to endogenous myf5 expression. Later, the GFP signals persist in caudal somites near the tail bud but are down-regulated in the older, rostral somites. During the pharyngula period, we detect GFP signals in pectoral fin buds, dorsal rostral myotomes, hypaxial myotomes, and inferior oblique and superior oblique muscles, a pattern that also corresponds well with endogenous myf5 transcripts. To characterize the specific upstream cis-elements that regulate this complex and dynamic expression pattern, we also generated several transgenic lines that harbor various lengths within the upstream 80-kb segment. We find that (1) the -80 kb/-9977 segment contains a fin and cranial muscle element and a notochord repressor; (2) the -9977/-6213 segment contains a strong repressive element that does not include the notochord-specific repressor; (3) the -6212/-2938 segment contains tissue-specific elements for bone and spinal cord; (4) the -2937/-291 segment contains an eye enhancer, and the -2937/-2457 segment is required for notochord and myocyte expression; and (5) the -290/-1 segment is responsible for basal transcription in somites and the presomitic mesoderm. CONCLUSION: We suggest that the cell lineage-specific expression of myf5 is delicately orchestrated by multiple modules within the distal upstream region. This study provides an insight to understand the molecular control of myf5 and myogenesis in the zebrafish.

Multiple upstream modules regulate zebrafish myf5 expression. Publishing Authors By Initials

YH Chen,YH Wang,MY Chang,CY Lin,CW Weng,M Westerfield,HJ Tsai,

For similar proteins: fish proteins: zebrafish proteins research abstracts see: proteins: fish proteins: zebrafish proteins research

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Multiple upstream modules regulate zebrafish myf5 expression. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: BMC developmental biology

VOLUME: 7

Page Numbers: 1

Journal Abbreviation: BMC Dev. Biol.

ISSN: 1471-213X

DAY: 3

MONTH: 01

YEAR: 2007

Multiple upstream modules regulate zebrafish myf5 expression. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100966973

Multiple upstream modules regulate zebrafish myf5 expression. Keywords Mesh Terms:

KEYWORDS: Zebrafish Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: Multiple upstream modules regulate zebrafish myf5 expression. Information

Substance Name: Zebrafish Proteins

Registry Number: 0

Grant and Affiliation Information for Multiple upstream modules regulate zebrafish myf5 expression.

AFFILIATION: Graduate Institute of Life Sciences, Tamkang University, Tamsui, Taiwan. yauhung@mail.tku.edu.tw <yauhung@mail.tku.edu.tw>

Country: England

AGENCY: United States NICHD

GRANT: HD22486

ACRONYM: HD

MEDLINETA: BMC Dev Biol

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