Multiple signaling pathways elicit circadian gene expression in cultured Rat-1 fibroblasts.

Multiple signaling pathways elicit circadian gene expression in cultured Rat-1 fibroblasts. Research Abstract Details 

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Multiple signaling pathways elicit circadian gene expression in cultured Rat-1 fibroblasts. Abstract Text:

A Balsalobre,L Marcacci,U Schibler,

In mammals, all overt circadian rhythms are thought to be coordinated by a central pacemaker residing in the hypothalamic suprachiasmatic nucleus (SCN) [1]. The phase of this pacemaker is entrained by photic cues via the retino-hypothalamic tract. Circadian clocks probably rely on a feedback loop in the expression of certain clock genes (reviewed in [2,3]). Surprisingly, however, such molecular oscillators are not only operative in pacemaker cells, such as SCN neurons, but also in many peripheral tissues and even in cell lines kept in vitro [4-7]. For example, a serum shock can induce circadian gene expression in cultured Rat-1 fibroblasts [5]. This treatment also results in a rapid surge of expression of the clock genes Per1 and Per2, similar to that observed in the SCNs of animals receiving a light pulse [8-10]. Serum induction of Per1 and Per2 transcription does not require ongoing protein synthesis [5] and must therefore be accomplished by direct signaling pathways. Here, we show that cAMP, protein kinase C, glucocorticoid hormones and Ca2+ can all trigger a transient surge of Per1 transcription and elicit rhythmic gene expression in Rat-1 cells. We thus suspect that the SCN pacemaker may exploit multiple chemical cues to synchronize peripheral oscillators in vivo.

Multiple signaling pathways elicit circadian gene expression in cultured Rat-1 fibroblasts. Publishing Authors By Initials

A Balsalobre,L Marcacci,U Schibler,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

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Multiple signaling pathways elicit circadian gene expression in cultured Rat-1 fibroblasts. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Current biology : CB

VOLUME: 10

Page Numbers: 1291-4

Journal Abbreviation: Curr. Biol.

ISSN: 0960-9822

DAY: 19

MONTH: Oct

YEAR: 2000

Multiple signaling pathways elicit circadian gene expression in cultured Rat-1 fibroblasts. Information

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LANGUAGE: eng

NlmUniqueID: 9107782

Multiple signaling pathways elicit circadian gene expression in cultured Rat-1 fibroblasts. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: drug effects

Chemical & Substance for Abstract: Multiple signaling pathways elicit circadian gene expression in cultured Rat-1 fibroblasts. Information

Substance Name: Okadaic Acid

Registry Number: 78111-17-8

Grant and Affiliation Information for Multiple signaling pathways elicit circadian gene expression in cultured Rat-1 fibroblasts.

AFFILIATION: Département de Biologie Moléculaire, Sciences II, Université de Genève, Switzerland.

Country: ENGLAND

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MEDLINETA: Curr Biol

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