Multiple phospholipase activation by OX(1) orexin/hypocretin receptors.

Multiple phospholipase activation by OX(1) orexin/hypocretin receptors. Research Abstract Details 

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Multiple phospholipase activation by OX(1) orexin/hypocretin receptors. Abstract Text:

We investigated coupling of OX(1) receptors to phospholipase activation and diacylglycerol generation in Chinese hamster ovary (CHO) cells using both biochemical and fluorescence "real-time" methods. The results indicate that at lowest orexin-A concentrations (highest potency), diacylglycerol generated results from phospholipase D activity. At 10-100-fold higher orexin-A concentrations, phospholipase C is activated, likely hydrolyzing phosphatidylinositol (PI) or phosphatidylinositol monophosphate (PIP) but not phosphatidylinositol bisphosphate (PIP(2)). At further 7-fold higher orexin-A concentrations, PIP(2) is hydrolyzed, releasing both diacylglycerol and inositol-1,4,5-trisphosphate. Thus, OX(1) orexin receptors connect to multiple phospholipase activities, apparently composed of at least one phospholipase D and two different phospholipase C activities. At low agonist concentrations, diacylglycerol and phosphatidic acid are the preferred products, and interestingly, it seems that even the primarily activated phospholipase C mainly works to increase diacylglycerol and not inositol-1,4,5-trisphosphate.

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Multiple phospholipase activation by OX(1) orexin/hypocretin receptors. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Cellular and molecular life sciences : CMLS

VOLUME: 65

Page Numbers: 1948-56

Journal Abbreviation: Cell. Mol. Life Sci.

ISSN: 1420-682X

DAY: 19

MONTH: Jun

YEAR: 2008

Multiple phospholipase activation by OX(1) orexin/hypocretin receptors. Information

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LANGUAGE: eng

NlmUniqueID: 9705402

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Grant and Affiliation Information for Multiple phospholipase activation by OX(1) orexin/hypocretin receptors.

AFFILIATION: Department of Neuroscience, Unit of Physiology, Uppsala University, Uppsala, Sweden.

Country: Switzerland

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MEDLINETA: Cell Mol Life Sci

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