Multiple pathways of selected gene amplification during adaptive mutation.

Multiple pathways of selected gene amplification during adaptive mutation. Research Abstract Details 

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Multiple pathways of selected gene amplification during adaptive mutation. Abstract Text:

Elisabeth Kugelberg,Eric Kofoid,Andrew B Reams,Dan I Andersson,John R Roth,

In a phenomenon referred to as "adaptive mutation," a population of bacterial cells with a mutation in the lac operon (lac-) accumulates Lac+ revertants during prolonged exposure to selective growth conditions (lactose). Evidence was provided that selective conditions do not increase the mutation rate but instead favor the growth of rare cells with a duplication of the leaky lac allele. A further increase in copy number (amplification) improves growth and increases the likelihood of a sequence change by adding more mutational targets to the clone (cells and lac copies per cell). These duplications and amplifications are described here. Before selection, cells with large (134-kb) lac duplications and long junction sequences (>1 kb) were common (0.2%). The same large repeats were found after selection in cells with a low-copy-number lac amplification. Surprisingly, smaller repeats (average, 34 kb) were found in high-copy-number amplifications. The small-repeat duplications form when deletions modify a preexisting large-repeat duplication. The shorter repeat size allowed higher lac amplification and better growth on lactose. Thus, selection favors a succession of gene-amplification types that make sequence changes more probable by adding targets. These findings are relevant to genetic adaptation in any biological systems in which fitness can be increased by adding gene copies (e.g., cancer and bacterial drug resistance).

Multiple pathways of selected gene amplification during adaptive mutation. Publishing Authors By Initials

E Kugelberg,E Kofoid,AB Reams,DI Andersson,JR Roth,

For similar genetic processes: selection (genetics) research abstracts see: genetic processes: selection (genetics) research

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Multiple pathways of selected gene amplification during adaptive mutation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 103

Page Numbers: 17319-24

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 2

MONTH: 11

YEAR: 2006

Multiple pathways of selected gene amplification during adaptive mutation. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Multiple pathways of selected gene amplification during adaptive mutation. Keywords Mesh Terms:

KEYWORDS: Selection (Genetics)

MESH TERMS: genetics

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Grant and Affiliation Information for Multiple pathways of selected gene amplification during adaptive mutation.

AFFILIATION: Section of Microbiology, College of Biological Sciences, University of California, Davis, CA 95616, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM27068

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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