Multiple glycolytic enzymes are tightly bound to the fibrous sheath of mouse spermatozoa.

Multiple glycolytic enzymes are tightly bound to the fibrous sheath of mouse spermatozoa. Research Abstract Details 

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Multiple glycolytic enzymes are tightly bound to the fibrous sheath of mouse spermatozoa. Abstract Text:

Michelle Krisfalusi,Kiyoshi Miki,Patricia L Magyar,Deborah A O'Brien,

The fibrous sheath is a cytoskeletal structure located in the principal piece of mammalian sperm flagella. Previous studies showed that glyceraldehyde 3-phosphate dehydrogenase, spermatogenic (GAPDHS), a germ cell-specific glycolytic isozyme that is required for sperm motility, is tightly bound to the fibrous sheath. To determine if other glycolytic enzymes are also bound to this cytoskeletal structure, we isolated highly purified fibrous sheath preparations from mouse epididymal sperm using a sequential extraction procedure. The isolated fibrous sheaths retain typical ultrastructural features and exhibit little contamination by axonemal or outer dense fiber proteins in Western blot analyses. Proteomic analysis using peptide-mass fingerprinting and MS/MS peptide fragment ion matching identified GAPDHS and two additional glycolytic enzyme subunits, the A isoform of aldolase 1 (ALDOA) and lactate dehydrogenase A (LDHA), in isolated fibrous sheaths. The presence of glycolytic enzymes in the fibrous sheath was also examined by Western blotting. In addition to GAPDHS, ALDOA, and LDHA, this method determined that pyruvate kinase is also tightly bound to the fibrous sheath. These data support a role for the fibrous sheath as a scaffold for anchoring multiple glycolytic enzymes along the length of the flagellum to provide a localized source of ATP that is essential for sperm motility.

Multiple glycolytic enzymes are tightly bound to the fibrous sheath of mouse spermatozoa. Publishing Authors By Initials

M Krisfalusi,K Miki,PL Magyar,DA O'Brien,

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Multiple glycolytic enzymes are tightly bound to the fibrous sheath of mouse spermatozoa. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biology of reproduction

VOLUME: 75

Page Numbers: 270-8

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ISSN: 0006-3363

DAY: 10

MONTH: 05

YEAR: 2006

Multiple glycolytic enzymes are tightly bound to the fibrous sheath of mouse spermatozoa. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Multiple glycolytic enzymes are tightly bound to the fibrous sheath of mouse spermatozoa.

AFFILIATION: Laboratory for Reproductive Biology, University of North Carolina School of Medicine, NC 27599, USA.

Country: United States

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MEDLINETA: Biol Reprod

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