Multiple genomic alterations on 21q22 predict various TMPRSS2/ERG fusion transcripts in human prostate cancers.

Multiple genomic alterations on 21q22 predict various TMPRSS2/ERG fusion transcripts in human prostate cancers. Research Abstract Details 

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Multiple genomic alterations on 21q22 predict various TMPRSS2/ERG fusion transcripts in human prostate cancers. Abstract Text:

Wennuan Liu,Charles M Ewing,Bao-Li Chang,Tao Li,Jishan Sun,Aubrey R Turner,Latchezar Dimitrov,Yi Zhu,Jielin Sun,Jin Woo Kim,S Lilly Zheng,William B Isaacs,Jianfeng Xu,

A number of TMPRSS2/ERG fusion transcripts have been reported since the discovery that recurrent genomic rearrangements result in the fusion of TMPRSS2 and ETS family member genes. In this article we present evidence demonstrating that multiple genomic alterations contribute to the formation of various TMPRSS2/ERG transcripts. Using allele-specific analysis of the data generated from the GeneChip 500K SNP array we observed both hemizygous and homozygous deletions occurring at different locations between and within TMPRSS2 and ERG in prostate cancers. The 500K SNP array enabled us to fine map the start and end of each deletion to specific introns of these two genes, and to predict a variety of fusion transcripts, including a new form which was confirmed by sequence analysis of the fusion transcripts in various tumors. We also inferred that translocation is an additional mechanism of fusion for these two genes in some tumors, based on largely diploid genomic DNA between TMPRSS and ERG, and different fusion transcripts produced in these tumors. Using a bioinformatics approach, we then uncovered the consensus sequences in the regions harboring the breakpoints of the deletions. These consensus sequences were homologous to the human Alu-Sq and Alu-Sp subfamily consensus sequences, with more than 80% homology. The presence/absence of Alu family consensus sequence in the introns of TMPRSS2 and ERG correlates with the presence/absence of fusion transcripts of theses two genes, indicating that these consensus sequences may contribute to genomic deletions and the fusion of TMPRSS2 and ERG in prostate cancer.

Multiple genomic alterations on 21q22 predict various TMPRSS2/ERG fusion transcripts in human prostate cancers. Publishing Authors By Initials

W Liu,CM Ewing,BL Chang,T Li,J Sun,AR Turner,L Dimitrov,Y Zhu,J Sun,JW Kim,SL Zheng,WB Isaacs,J Xu,

For similar proteins: dna-binding proteins: trans-activators research abstracts see: proteins: dna-binding proteins: trans-activators research

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Multiple genomic alterations on 21q22 predict various TMPRSS2/ERG fusion transcripts in human prostate cancers. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Genes, chromosomes & cancer

VOLUME: 46

Page Numbers: 972-80

Journal Abbreviation: Genes Chromosomes Cancer

ISSN: 1045-2257

DAY: 3

MONTH: Nov

YEAR: 2007

Multiple genomic alterations on 21q22 predict various TMPRSS2/ERG fusion transcripts in human prostate cancers. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9007329

Multiple genomic alterations on 21q22 predict various TMPRSS2/ERG fusion transcripts in human prostate cancers. Keywords Mesh Terms:

KEYWORDS: Trans-Activators

MESH TERMS: genetics

Chemical & Substance for Abstract: Multiple genomic alterations on 21q22 predict various TMPRSS2/ERG fusion transcripts in human prostate cancers. Information

Substance Name: TMPRSS2 protein, human

Registry Number: EC 3.4.21.-

Grant and Affiliation Information for Multiple genomic alterations on 21q22 predict various TMPRSS2/ERG fusion transcripts in human prostate cancers.

AFFILIATION: Center for Human Genomics, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.

Country: United States

AGENCY: United States NCI

GRANT: CA95052

ACRONYM: CA

MEDLINETA: Genes Chromosomes Cancer

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