Multiple forms of staphylococcal alpha-toxin.

Multiple forms of staphylococcal alpha-toxin. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Multiple forms of staphylococcal alpha-toxin. Abstract Text:

A group of proteins was readily extracted at neutrality from trichloroacetic acid precipitates of staphylococcal culture filtrate supernatants, while alpha-toxin was dissolved and activated by treating the precipitate with 8 M urea, with acidic buffers or by heating to 90-100 degrees C at neutrality. Heat activation of the precipitate produced a relatively pure alpha-toxin with a molecular weight of 39,000. alpha-Toxin was eluted together with three other proteins on hydroxyl apatite chromatography, and evidence was obtained for an association between the four proteins. On isoelectric focusing a haemolytic fraction was obtained at pH 6.2, probably due to acid activation of the precipitate formed at the cathodic end of the column. The alpha-haemolytic fractions with pI's of 7.4 and 8.6 were shown to consist of alpha-toxin only when analyzed by acrylamide electrophoresis in the presence of sodium dodecyl sulphate. The haemolytic component with a pI of 9.2 contained two additional components of molecular weights of 27,500 and 18,000. Chromatography of this material on Sephadex G-200 showed that alpha-toxin and the two proteins appeared as a high molecular complex.

Multiple forms of staphylococcal alpha-toxin. Publishing Authors By Initials

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

PUBMED ID PMID:

MEDLINE DATE:

Multiple forms of staphylococcal alpha-toxin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta pathologica et microbiologica Scandinavica. S

VOLUME: 83

Page Numbers: 561-8

Journal Abbreviation:

ISSN:

DAY: 26

MONTH: Dec

YEAR: 1975

Multiple forms of staphylococcal alpha-toxin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8211440

Multiple forms of staphylococcal alpha-toxin. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Multiple forms of staphylococcal alpha-toxin. Information

Substance Name: Urea

Registry Number: 57-13-6

Grant and Affiliation Information for Multiple forms of staphylococcal alpha-toxin.

AFFILIATION:

Country: DENMARK

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Acta Pathol Microbiol Scand Su

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Multiple forms of staphylococcal alpha-toxin Related Publications

• Characterization of virus-like particles from a psoriatic patient with respect to the possible presence of particle-associated RNA and RNA-directed DNA polymerase.
• Coexpression of c-erbB 1-4 receptor proteins in human glioblastomas. An immunohistochemical study.
• Dichotic listening, forced attention, and brain asymmetry in righthanded and lefthanded children.
• Is aspirin resistance due to noncompliance?
• Itch in the community: associations with psychosocial factors among adults.
• Middle ear mucosa in cleft palate children. A scanning electron microscopic study.
• Month of birth and schizophrenia.
• Multiple forms of staphylococcal alpha-toxin.
• Pharmacologically inappropriate prescriptions for elderly patients in general practice: How common?
• Should patients with venous thromboembolism be screened for thrombophilia?
• The moratorium on US medical school enrollment, from 1980 to 2005: what were we thinking?
• The use of homocarnosine as a spacer in indirect haemagglutination.
• Urine proteins cross-reacting with antiserum against virus-like particles from cultured psoriatic epithelial cells.
• Venous thromboembolism and the archives of internal medicine, 1908 to 2005.
• [Chlamydia trachomatis and ectopic pregnancy. A causal relationship]
• [Chlamydia trachomatis and infertility in women]
• [Chlamydia trachomatis in cervix uteri specimens among patients in general practice]
• [Chlamydia trachomatis in induced abortion]
• [Encephalitis after acute Epstein-Barr virus infection]
• [Mechanisms of persistent viral infections]
• [Mycoplasma pneumoniae infection]
• [Obesity. Treatment with intermaxillary fixation and diet]