Multiple controls regulate nucleostemin partitioning between nucleolus and nucleoplasm.

Multiple controls regulate nucleostemin partitioning between nucleolus and nucleoplasm. Research Abstract Details 

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Multiple controls regulate nucleostemin partitioning between nucleolus and nucleoplasm. Abstract Text:

Lingjun Meng,Hiroaki Yasumoto,Robert Y L Tsai,

Nucleostemin plays an essential role in maintaining the continuous proliferation of stem cells and cancer cells. The movement of nucleostemin between the nucleolus and the nucleoplasm provides a dynamic way to partition the nucleostemin protein between these two compartments. Here, we show that nucleostemin contains two nucleolus-targeting regions, the basic and the GTP-binding domains, that exhibit a short and a long nucleolar retention time, respectively. In a GTP-unbound state, the nucleolus-targeting activity of nucleostemin is blocked by a mechanism that traps its intermediate domain in the nucleoplasm. A nucleostemin-interacting protein, RSL1D1, was identified that contains a ribosomal L1-domain. RSL1D1 co-resides with nucleostemin in the same subnucleolar compartment, unlike the B23 and fibrillarin, and displays a longer nucleolar residence time than nucleostemin. It interacts with both the basic and the GTP-binding domains of nucleostemin through a non-nucleolus-targeting region. Overexpression of the nucleolus-targeting domain of RSL1D1 alone disperses nucleolar nucleostemin. Loss of RSL1D1 expression reduces the compartmental size and amount of nucleostemin in the nucleolus. Our work reveals that the partitioning of nucleostemin employs complex mechanisms involving both nucleolar and nucleoplasmic components, and provides insight into the post-translational regulation of its activity.

Multiple controls regulate nucleostemin partitioning between nucleolus and nucleoplasm. Publishing Authors By Initials

L Meng,H Yasumoto,RY Tsai,

For similar investigative techniques: genetic techniques: cloning, molecular: two-hybrid system techniques research abstracts see: investigative techniques: genetic techniques: cloning, molecular: two-hybrid system techniques research

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Multiple controls regulate nucleostemin partitioning between nucleolus and nucleoplasm. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of cell science

VOLUME: 119

Page Numbers: 5124-36

Journal Abbreviation: J. Cell. Sci.

ISSN: 0021-9533

DAY: 15

MONTH: Dec

YEAR: 2006

Multiple controls regulate nucleostemin partitioning between nucleolus and nucleoplasm. Information

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LANGUAGE: eng

NlmUniqueID: 52457

Multiple controls regulate nucleostemin partitioning between nucleolus and nucleoplasm. Keywords Mesh Terms:

KEYWORDS: Two-Hybrid System Techniques

MESH TERMS: metabolism

Chemical & Substance for Abstract: Multiple controls regulate nucleostemin partitioning between nucleolus and nucleoplasm. Information

Substance Name: Nuclear Proteins

Registry Number: 0

Grant and Affiliation Information for Multiple controls regulate nucleostemin partitioning between nucleolus and nucleoplasm.

AFFILIATION: Center for Cancer and Stem Cell Biology, Alkek Institute of Biosciences and Technology, Texas A&M Health Science Center, 2121 W Holcombe Blvd, Houston, TX 77030-3303, USA.

Country: England

AGENCY: United States NCI

GRANT: R01 CA113750-01

ACRONYM: CA

MEDLINETA: J Cell Sci

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