Multiple actin-related proteins of Saccharomyces cerevisiae are present in the nucleus.

Multiple actin-related proteins of Saccharomyces cerevisiae are present in the nucleus. Research Abstract Details 

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Multiple actin-related proteins of Saccharomyces cerevisiae are present in the nucleus. Abstract Text:

M Harata,Y Oma,T Tabuchi,Y Zhang,D J Stillman,S Mizuno,

An increasing number of actin-related proteins (Arps), which share the basal structure with skeletal actin but possess distinct functions, have been found in a wide variety of organisms. Individual Arps of Saccharomyces cerevisiae were classified into Arps 1-10 based on the relatedness of their sequences and functions, where Arp1 is the most similar to actin, and Arp10 is the least similar. While Arps 1-3 and their orthologs in other organisms are localized exclusively in the cytoplasm, Arp4 (also known as Act3) is localized in the nucleus and is involved in transcriptional regulation. Here we examined the more divergent Arps for possible nuclear functions. We show that Arps 5-9 are localized in the nucleus, but Arp10 is not. The nuclear export signals identified in actin are well conserved in the cytoplasmic Arps, Arps 1-3, but less conserved in the nuclear Arps. Gel filtration chromatography experiments show that the nuclear Arps are larger than monomer in size and thus are present in multi-protein complexes. Since nuclear protein complexes containing Arps are found to be responsible for histone acetylation and chromatin remodeling, it is suggested that most of the divergent Arps are involved in the !transcriptional regulation through chromatin modulation.

Multiple actin-related proteins of Saccharomyces cerevisiae are present in the nucleus. Publishing Authors By Initials

M Harata,Y Oma,T Tabuchi,Y Zhang,DJ Stillman,S Mizuno,

For similar cells: cellular structures: subcellular fractions research abstracts see: cells: cellular structures: subcellular fractions research

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Multiple actin-related proteins of Saccharomyces cerevisiae are present in the nucleus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 128

Page Numbers: 665-71

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 2000

Multiple actin-related proteins of Saccharomyces cerevisiae are present in the nucleus. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Multiple actin-related proteins of Saccharomyces cerevisiae are present in the nucleus. Keywords Mesh Terms:

KEYWORDS: Subcellular Fractions

MESH TERMS: chemistry

Chemical & Substance for Abstract: Multiple actin-related proteins of Saccharomyces cerevisiae are present in the nucleus. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for Multiple actin-related proteins of Saccharomyces cerevisiae are present in the nucleus.

AFFILIATION: Laboratory of Molecular Biology, Department of Molecular and Cell Biology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan. mharata@biochem.tohoku.ac.jp

Country: JAPAN

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MEDLINETA: J Biochem

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