Multiphoton laser-scanning microscopy and spatial analysis of dehydroergosterol distributions on plasma membrane of living cells.

Multiphoton laser-scanning microscopy and spatial analysis of dehydroergosterol distributions on plasma membrane of living cells. Research Abstract Details 

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Multiphoton laser-scanning microscopy and spatial analysis of dehydroergosterol distributions on plasma membrane of living cells. Abstract Text:

Avery L McIntosh,Barbara P Atshaves,Huan Huang,Adalberto M Gallegos,Ann B Kier,Friedhelm Schroeder,Hai Xu,Weimin Zhang,Suojin Wang,Jyh-Charn Liu,Avery L McIntosh,Barbara P Atshaves,Huan Huang,Adalberto M Gallegos,Ann B Kier,Friedhelm Schroeder,Hai Xu,Weimin Zhang,Suojin Wang,Jyh-Charn Liu,

Multiphoton laser-scanning microscopy (MPLSM) imaging in combination with advanced image analysis techniques provides unique opportunities to visualize the arrangement of cholesterol in the plasma membrane (PM) of living cells. MPLSM makes possible the use of a naturally occurring sterol, dehydroergosterol (DHE), for observing sterol-enriched areas of the PM. Pure DHE has properties similar to cholesterol as observed in model and cellular membranes but with a conjugated double-bond system that fluoresces at ultraviolet wavelengths. MPLSM enables the excitation of DHE at infrared wavelengths that many laser-scanning microscopy systems are able to transmit effectively and that are less harmful to the cell. Thus, with the incorporation of DHE into living cells and the advent of MPLSM, real-time images of the cellular distribution of DHE can be obtained. In juxtaposition, notably the application of newly advanced techniques in image analysis, aids not only the identification and segmentation of sterol-rich regions of the PM of cells, but also the elucidation of the statistical nature of the observed patterns. In studies involving murine L-cell (Larpt-+K-) fibroblasts, DHE is shown to exhibit strong cluster patterns within the PM.

Multiphoton laser-scanning microscopy and spatial analysis of dehydroergosterol distributions on plasma membrane of living cells. Publishing Authors By Initials

AL McIntosh,BP Atshaves,H Huang,AM Gallegos,AB Kier,F Schroeder,H Xu,W Zhang,S Wang,JC Liu,AL McIntosh,BP Atshaves,H Huang,AM Gallegos,AB Kier,F Schroeder,H Xu,W Zhang,S Wang,JC Liu,

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Multiphoton laser-scanning microscopy and spatial analysis of dehydroergosterol distributions on plasma membrane of living cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 398

Page Numbers: 85-105

Journal Abbreviation: Methods Mol. Biol.

ISSN: 1064-3745

DAY: 24

MONTH: 01

YEAR: 2007

Multiphoton laser-scanning microscopy and spatial analysis of dehydroergosterol distributions on plasma membrane of living cells. Information

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LANGUAGE: eng

NlmUniqueID: 9214969

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Chemical & Substance for Abstract: Multiphoton laser-scanning microscopy and spatial analysis of dehydroergosterol distributions on plasma membrane of living cells. Information

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Grant and Affiliation Information for Multiphoton laser-scanning microscopy and spatial analysis of dehydroergosterol distributions on plasma membrane of living cells.

AFFILIATION: Dept. of Physiology and Pharmacology, Texas A & M University, College Station 77843, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: P20 GM72041

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MEDLINETA: Methods Mol Biol

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