Multiparametric analysis of normal and postchemotherapy bone marrow: Implication for the detection of leukemia-associated immunophenotypes.

Multiparametric analysis of normal and postchemotherapy bone marrow: Implication for the detection of leukemia-associated immunophenotypes. Research Abstract Details 

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Multiparametric analysis of normal and postchemotherapy bone marrow: Implication for the detection of leukemia-associated immunophenotypes. Abstract Text:

D Olaru,L Campos,P Flandrin,N Nadal,A Duval,S Chautard,D Guyotat,D Olaru,L Campos,P Flandrin,N Nadal,A Duval,S Chautard,D Guyotat,D Olaru,L Campos,P Flandrin,N Nadal,A Duval,S Chautard,D Guyotat,

BACKGROUND:: The knowledge of normal marrow is mandatory to assess the malignant counterpart of normal cells and define leukemia-associated immunophenotypes (LAIPs). In this study, the expression of a variety of antigens expressed in normal and postchemotherapy bone marrow (BM) was analyzed to provide a frame of reference for the identification of myeloid LAIPs. METHODS:: Multiparameter four- and six-color flow cytometry was used to define antigen combinations totally absent or present at very minimal levels in marrow cells of normal individuals (n = 20) and patients receiving chemotherapy for acute lymphoblastic leukemia (n = 20). Immature (blast) cells were gated according to CD45/SSC properties. Fifty-three acute myeloid leukemia (AML) samples were studied in six-color combinations. RESULTS:: In six-color flow cytometry, 47 phenotypes were totally absent from blast gate in all normal samples. Forty-one other phenotypes were identified in less than 0.05% of blast cells. There was no difference between normal and postchemotherapy BMs. The four-color panel allowed to identify only 30 phenotypes present at a frequency <0.05%. Using the six-color panel, 58% of the absent or infrequent phenotypes in normal BM were found in at least one of 53 AML samples. All AML cases exhibited at least one LAIP. CONCLUSION:: Our results show that the ability to distinguish leukemic from healthy cells is considerably increased by a six-color approach. Furthermore, these absent or infrequent phenotypes in normal BM are identified in AML and can be utilized for minimal residual disease study. (c) 2007 Clinical Cytometry Society.

Multiparametric analysis of normal and postchemotherapy bone marrow: Implication for the detection of leukemia-associated immunophenotypes. Publishing Authors By Initials

D Olaru,L Campos,P Flandrin,N Nadal,A Duval,S Chautard,D Guyotat,D Olaru,L Campos,P Flandrin,N Nadal,A Duval,S Chautard,D Guyotat,D Olaru,L Campos,P Flandrin,N Nadal,A Duval,S Chautard,D Guyotat,

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Multiparametric analysis of normal and postchemotherapy bone marrow: Implication for the detection of leukemia-associated immunophenotypes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Cytometry. Part B, Clinical cytometry

VOLUME: 74

Page Numbers: 17-24

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ISSN: 1552-4949

DAY: 24

MONTH: Jan

YEAR: 2008

Multiparametric analysis of normal and postchemotherapy bone marrow: Implication for the detection of leukemia-associated immunophenotypes. Information

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LANGUAGE: eng

NlmUniqueID: 101235690

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AFFILIATION: Laboratoire d'Hématologie Hôpital Nord, CHU de Saint-Etienne, France.

Country: United States

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MEDLINETA: Cytometry B Clin Cytom

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