Multilocus sequence typing compared to pulsed-field gel electrophoresis for molecular typing of Pseudomonas aeruginosa.

Multilocus sequence typing compared to pulsed-field gel electrophoresis for molecular typing of Pseudomonas aeruginosa. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Multilocus sequence typing compared to pulsed-field gel electrophoresis for molecular typing of Pseudomonas aeruginosa. Abstract Text:

Jennifer K Johnson,Sonia M Arduino,O Colin Stine,Judith A Johnson,Anthony D Harris,Jennifer K Johnson,Sonia M Arduino,O Colin Stine,Judith A Johnson,Anthony D Harris,

For hospital epidemiologists, determining a system of typing that is discriminatory is essential for measuring the effectiveness of infection control measures. In situations in which the incidence of resistant Pseudomonas aeruginosa is increasing, the ability to discern whether it is due to patient-to-patient transmission versus an increase in patient endogenous strains is often made on the basis of molecular typing. The present study compared the discriminatory abilities of pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) for 90 P. aeruginosa isolates obtained from cultures of perirectal surveillance swabs from patients in an intensive care unit. PFGE identified 85 distinct types and 76 distinct groups when similarity cutoffs of 100% and 87%, respectively, were used. By comparison, MLST identified 60 sequence types that could be clustered into 11 clonal complexes and 32 singletons. By using the Simpson index of diversity (D), PFGE had a greater discriminatory ability than MLST for P. aeruginosa isolates (D values, 0.999 versus 0.975, respectively). Thus, while MLST was better for detecting genetic relatedness, we determined that PFGE was more discriminatory than MLST for determining genetic differences in P. aeruginosa.

Multilocus sequence typing compared to pulsed-field gel electrophoresis for molecular typing of Pseudomonas aeruginosa. Publishing Authors By Initials

JK Johnson,SM Arduino,OC Stine,JA Johnson,AD Harris,JK Johnson,SM Arduino,OC Stine,JA Johnson,AD Harris,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Multilocus sequence typing compared to pulsed-field gel electrophoresis for molecular typing of Pseudomonas aeruginosa. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of clinical microbiology

VOLUME: 45

Page Numbers: 3707-12

Journal Abbreviation: J. Clin. Microbiol.

ISSN: 0095-1137

DAY: 19

MONTH: 09

YEAR: 2007

Multilocus sequence typing compared to pulsed-field gel electrophoresis for molecular typing of Pseudomonas aeruginosa. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505564

Multilocus sequence typing compared to pulsed-field gel electrophoresis for molecular typing of Pseudomonas aeruginosa. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Multilocus sequence typing compared to pulsed-field gel electrophoresis for molecular typing of Pseudomonas aeruginosa. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Multilocus sequence typing compared to pulsed-field gel electrophoresis for molecular typing of Pseudomonas aeruginosa.

AFFILIATION: Department of Pathology, University of Maryland School of Medicine, MSTF, Baltimore, MD 21201, USA. jkjohnson@som.umaryland.edu

Country: United States

AGENCY: United States NIAID

GRANT: R01 AI60859-01A1

ACRONYM: AI

MEDLINETA: J Clin Microbiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Multilocus sequence typing compared to pulsed-field gel electrophoresis for molecular typing of Pseudomonas aeruginosa Related Publications

• A clonal chondrocytic cell line derived from BMP-2/T antigen-expressing transgenic mouse.
• Activation of oxygen nucleophiles in enzyme catalysis.
• Autoregulation of mouse BMP-2 gene transcription is directed by the proximal promoter element.
• Biomechanical implications of the negative heel rocker sole shoe: gait kinematics and kinetics.
• Bone morphogenetic protein 2 (BMP-2) enhances BMP-3, BMP-4, and bone cell differentiation marker gene expression during the induction of mineralized bone matrix formation in cultures of fetal rat calvarial osteoblasts.
• Bone morphogenetic protein 2 transcripts in rapidly developing deer antler tissue contain an extended 5' non-coding region arising from a distal promoter.
• CFTR expression does not influence glycosylation of an epitope-tagged MUC1 mucin in colon carcinoma cell lines.
• COX-2 and the kidney.
• Clonal osteoblastic cell lines from p53 null mouse calvariae are immortalized and dependent on bone morphogenetic protein 2 for mature osteoblastic phenotype.
• Dense core vesicles resemble active-zone transport vesicles and are diminished following synaptogenesis in mature hippocampal slices.
• Diabetes, hyperglycemia, and inflammation in older individuals: the health, aging and body composition study.
• Diabetes, inflammation, and functional decline in older adults: findings from the Health, Aging and Body Composition (ABC) study.
• Differential roles for bone morphogenetic protein (BMP) receptor type IB and IA in differentiation and specification of mesenchymal precursor cells to osteoblast and adipocyte lineages.
• Exaggerated response to mild stress in rats fed high-fat diet.
• Gene expression profiling in osteoblast biology: bioinformatic tools.
• Genetics of body condition score in New Zealand dairy cows.
• Glycemia and cognitive function in older adults using glucose-lowering drugs.
• Gyrodactylid developmental biology: historical review, current status and future trends.
• Human APOBEC3 proteins, retrovirus restriction, and HIV drug resistance.
• Immunoregulation in pancreatic cancer patients.
• Impaired growth and neurological abnormalities in branched-chain alpha-keto acid dehydrogenase kinase-deficient mice.
• Leisure-time exercise and overall physical activity in older women caregivers and non-caregivers from the Caregiver-SOF Study.
• Plasticity of perisynaptic astroglia during synaptogenesis in the mature rat hippocampus.
• Pondermotive forces with slow light.
• Renal cortical cyclooxygenase 2 expression is differentially regulated by angiotensin II AT(1) and AT(2) receptors.
• Role of EGF receptor activation in angiotensin II-induced renal epithelial cell hypertrophy.
• The contribution of tandem repeat number to the O-glycosylation of mucins.
• Therapeutic Strategies for Localized Prostate Cancer II: Perineal Prostatectomy, X-Rays, Protons, Neutrons, and Combination Brachytherapy.
• Visualizing pancreatic beta-cell mass with [11C]DTBZ.
• c-erbB-2 and episialin challenge host immune response by HLA class I expression in human non-small-cell lung cancer.