Multicolor super-resolution imaging with photo-switchable fluorescent probes.

Multicolor super-resolution imaging with photo-switchable fluorescent probes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Multicolor super-resolution imaging with photo-switchable fluorescent probes. Abstract Text:

Mark Bates,Bo Huang,Graham T Dempsey,Xiaowei Zhuang,

Recent advances in far-field optical nanoscopy have enabled fluorescence imaging with a spatial resolution of 20 to 50 nanometers. Multicolor super-resolution imaging, however, remains a challenging task. Here, we introduce a family of photo-switchable fluorescent probes and demonstrate multicolor stochastic optical reconstruction microscopy (STORM). Each probe consists of a photo-switchable "reporter" fluorophore that can be cycled between fluorescent and dark states, and an "activator" that facilitates photo-activation of the reporter. Combinatorial pairing of reporters and activators allows the creation of probes with many distinct colors. Iterative, color-specific activation of sparse subsets of these probes allows their localization with nanometer accuracy, enabling the construction of a super-resolution STORM image. Using this approach, we demonstrate multicolor imaging of DNA model samples and mammalian cells with 20- to 30-nanometer resolution. This technique will facilitate direct visualization of molecular interactions at the nanometer scale.

Multicolor super-resolution imaging with photo-switchable fluorescent probes. Publishing Authors By Initials

M Bates,B Huang,GT Dempsey,X Zhuang,

For similar natural sciences: nanotechnology research abstracts see: natural sciences: nanotechnology research

PUBMED ID PMID:

MEDLINE DATE:

Multicolor super-resolution imaging with photo-switchable fluorescent probes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Science (New York, N.Y.)

VOLUME: 317

Page Numbers: 1749-53

Journal Abbreviation: Science

ISSN: 1095-9203

DAY: 16

MONTH: 08

YEAR: 2007

Multicolor super-resolution imaging with photo-switchable fluorescent probes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 404511

Multicolor super-resolution imaging with photo-switchable fluorescent probes. Keywords Mesh Terms:

KEYWORDS: Nanotechnology

MESH TERMS: methods

Chemical & Substance for Abstract: Multicolor super-resolution imaging with photo-switchable fluorescent probes. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Multicolor super-resolution imaging with photo-switchable fluorescent probes.

AFFILIATION: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM 068518

ACRONYM: GM

MEDLINETA: Science

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Multicolor super-resolution imaging with photo-switchable fluorescent probes Related Publications

• A New Method to Get More Robust MTF Curve.
• Biomedical imaging and image processing course in shanghai jiao tong university.
• Effects of Survivin expression suppressed by short hairpin RNA on MCF-7 cells.
• Kinetics of inhibition of beta-glucosidase from Ampullarium crossean by bromoacetic acid.
• [An introduction of a treatment algorithm for the management of chronic hepatitis B virus infection in the USA]
• [Case control study on risk factors of congenital microtia]
• [Characteristics of T cell receptors recognizing antiphospholipid syndrome associated antigens]
• [Classification and repairment of "butterfly ear" deformity]
• [Comprehensive quality appraisal of "maidong"]
• [Determination of naphthenic acids in distillates of crude oil by gas chromatography/chemical ionization-mass spectrometry]
• [Development of DICOM software platfrom for medical image communication]
• [Diagnosis of hereditary tyrosinemia type I: clinical study of ten patients]
• [Differentially express genes in human embryonic lung fibroblasts with damage tolerance induced by low-dose hydroquinone]
• [Endovascular stent-graft exclusion on aortic dissection and dissecting aneurysm]
• [Expert explanation of "The guideline of prevention and treatment for chronic hepatitis B"]
• [Features of morphometry of ultrasound and endometrial histology in the anovulatory polycystic ovary syndrome women]
• [Induction of free radicals after dietary exposure by mercury contaminated rice and protective effect of coexisting selenium]
• [Influence of substance P on the proliferation and apoptosis of fibroblasts of pathological scars]
• [Influence of the depth of embryo transfer on pregnancy outcome in in vitro fertilization-embryo transfer]
• [Research on testing the robustness of DICOM-e-mail secure teleradiology systems]
• [Research on the distributed PACS based on grid technology]
• [Safety analysis of dust mite allergen for diagnosis and immunotherapy of asthma and rhinitis]
• [Sperm sex chromosome analysis and preimplantation genetic diagnosis of patients with sex chromosome anomalies]
• [Stability of hepatitis C virus RNA in various processing and storage conditions]
• [Strengthen immunization of medical and health care workers against hepatitis B]
• [Studies on bilingual teaching of genetics]
• [The correlation of PDGF and TNF-alpha and HA and nasal mucosa healing after ESS]
• [The latest development in preimplantation genetic diagnosis.]
• [The study of associated reconstruction using MV linear accelerator and cone-beam CT]
• [Transcatheter closure of perimembrane ventricular septal defect using Amplatzer occluder device]